Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation

Version 1 : Received: 11 January 2021 / Approved: 12 January 2021 / Online: 12 January 2021 (15:13:01 CET)

A peer-reviewed article of this Preprint also exists.

Panteris, E.; Kouskouveli, A.; Pappas, D.; Adamakis, I.-D.S. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. Int. J. Mol. Sci. 2021, 22, 1405. Panteris, E.; Kouskouveli, A.; Pappas, D.; Adamakis, I.-D.S. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. Int. J. Mol. Sci. 2021, 22, 1405.

Journal reference: Int. J. Mol. Sci. 2021, 22, 1405
DOI: 10.3390/ijms22031405

Abstract

Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate, to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), while deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy, in root cells of the fra2 Arabidopsis thaliana mutant. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls appeared also faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild-type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild-type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

Subject Areas

Arabidopsis thaliana; callose; cell plate; cross wall; cytokinesis; homogalacturonans; katanin; KNOLLE syntaxin; microtubules; phragmoplast

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