Zykin, P.A.; Andreeva, E..A.; Tsvetkova, N.V.; Voylokov, A.V. Anatomical and Image Analysis of Grain Coloration in Rye. Preprints2020, 2020110530. https://doi.org/10.20944/preprints202011.0530.v1
Zykin, P.A., Andreeva, E..A., Tsvetkova, N.V., & Voylokov, A.V. (2020). Anatomical and Image Analysis of Grain Coloration in Rye. Preprints. https://doi.org/10.20944/preprints202011.0530.v1
Zykin, P.A., Natalia V. Tsvetkova and Anatoly V. Voylokov. 2020 "Anatomical and Image Analysis of Grain Coloration in Rye" Preprints. https://doi.org/10.20944/preprints202011.0530.v1
In rye, there is a considerable variety of grain color which is determined by the diversity of compounds localized in different parts of the grain (caryopsis) - pericarp, testa, and aleurone. The localization of anthocyanins and proanthocyanidins was analyzed in 26 rye samples with identified anthocyanin genes, along with the analysis of CIE color coordinates. The Grain Scan program  was used to analyze images of individual grains. The localization of anthocyanins and proanthocyanidins was studied on longitudinal and cross sections of grains using light microscopy and MALDI-imaging. The violet-grained samples contain anthocyanins in the pericarp, and the green-grained samples contain anthocyanins in the aleurone layer. The green, violet and yellow-grained rye, with the exception of two anthocyaninless mutants vi3 and vi6, shows the presence of proanthocyanidins in the brown-colored testa. Four main color groups of the rye grains (yellow, green, brown, violet) could be differentiated using the color coordinate h° (hue angle). Interspecies and intraspecies variability for the localization of colored flavonoids in cereal grains is discussed.
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