Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Anatomical and Image Analysis of Grain Coloration in Rye

Version 1 : Received: 18 November 2020 / Approved: 20 November 2020 / Online: 20 November 2020 (09:34:01 CET)

How to cite: Zykin, P.A.; Andreeva, E..A.; Tsvetkova, N.V.; Voylokov, A.V. Anatomical and Image Analysis of Grain Coloration in Rye. Preprints 2020, 2020110530 (doi: 10.20944/preprints202011.0530.v1). Zykin, P.A.; Andreeva, E..A.; Tsvetkova, N.V.; Voylokov, A.V. Anatomical and Image Analysis of Grain Coloration in Rye. Preprints 2020, 2020110530 (doi: 10.20944/preprints202011.0530.v1).

Abstract

In rye, there is a considerable variety of grain color which is determined by the diversity of compounds localized in different parts of the grain (caryopsis) - pericarp, testa, and aleurone. The localization of anthocyanins and proanthocyanidins was analyzed in 26 rye samples with identified anthocyanin genes, along with the analysis of CIE color coordinates. The Grain Scan program [1] was used to analyze images of individual grains. The localization of anthocyanins and proanthocyanidins was studied on longitudinal and cross sections of grains using light microscopy and MALDI-imaging. The violet-grained samples contain anthocyanins in the pericarp, and the green-grained samples contain anthocyanins in the aleurone layer. The green, violet and yellow-grained rye, with the exception of two anthocyaninless mutants vi3 and vi6, shows the presence of proanthocyanidins in the brown-colored testa. Four main color groups of the rye grains (yellow, green, brown, violet) could be differentiated using the color coordinate h° (hue angle). Interspecies and intraspecies variability for the localization of colored flavonoids in cereal grains is discussed.

Subject Areas

rye; image analysis; grain color; anthocyanins; proanthocyanidins

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