Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Photobiomodulation of human fibroblasts and keratinocytes with blue light: implications in wound healing

Version 1 : Received: 9 November 2020 / Approved: 12 November 2020 / Online: 12 November 2020 (09:13:12 CET)

How to cite: Rossi, F.; Magni, G.; Tatini, F.; Banchelli, M.; Cherchi, F.; Rossi, M.; Coppi, E.; Pugliese, A.M.; Rossi degl'Innocenti, D.; Alfieri, D.; Pavone, F.S.; Pini, R.; Matteini, P. Photobiomodulation of human fibroblasts and keratinocytes with blue light: implications in wound healing. Preprints 2020, 2020110332 (doi: 10.20944/preprints202011.0332.v1). Rossi, F.; Magni, G.; Tatini, F.; Banchelli, M.; Cherchi, F.; Rossi, M.; Coppi, E.; Pugliese, A.M.; Rossi degl'Innocenti, D.; Alfieri, D.; Pavone, F.S.; Pini, R.; Matteini, P. Photobiomodulation of human fibroblasts and keratinocytes with blue light: implications in wound healing. Preprints 2020, 2020110332 (doi: 10.20944/preprints202011.0332.v1).

Abstract

In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts and keratinocytes. Different light doses were used to treat the cells, evidencing inhibitory and stimulatory effects. Electrophysiology was used to investigate the effects on membrane currents, while Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation. In conclusion, we observed that the blue LED light can be used to modulate the activity of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

Subject Areas

photobiomodulation; blue light; LED; wound healing

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.