Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing
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A peer-reviewed article of this Preprint also exists.
Rossi, F.; Magni, G.; Tatini, F.; Banchelli, M.; Cherchi, F.; Rossi, M.; Coppi, E.; Pugliese, A.M.; Rossi degl’Innocenti, D.; Alfieri, D.; Pavone, F.S.; Pini, R.; Matteini, P. Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing. Biomedicines 2021, 9, 41. Rossi, F.; Magni, G.; Tatini, F.; Banchelli, M.; Cherchi, F.; Rossi, M.; Coppi, E.; Pugliese, A.M.; Rossi degl’Innocenti, D.; Alfieri, D.; Pavone, F.S.; Pini, R.; Matteini, P. Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing. Biomedicines 2021, 9, 41.
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Commenter: Gareth Stephen Hazell
The commenter has declared there is no conflict of interests.
It was very nice to read your paper and extremely interesting, congratulations on completeing it.
It was interesting to read that high doses of blue light promote a drop in metabolism and low doses a rise. I am new at looking at photo-biomodulation, and have recently started to try to do this in skin cells (keratinocytes, fibroblasts and endothelial cells from donor biopsies). My aim is to - like you, assess if the visible spectra can elicit some positive rejuvenateive effect via activation of chromophores.
I have been concentrating on blue light, with chronic irradiation, approximately 6J/cm2 per hour with a total dose of 45J over several hours. When I first started I was naive enough to use DMEM in FBS to irradiate the cells and found much to my surprise the cells had died (i now know this to be phenol red, porphyrin, flavins, folic acid and tryptophan in the media generating an artefact effect via ROS release.
I have come some way in countering this effect - via use of media without these constituents in over the irradiation period, and am in the final stages of testing. I was wondering how you did these irradiations - im guessing you did so in a similar fashion, via leaving the FBS or the serum based component out of the media while irradiating and the other factors i mentioned too - with this in mind I was wondering do you make your own media or purchase directly, At present I am useing the MEMO media from cell signalling systems, but used a modified L-15 in the past that seemed to promote the same effect as it had no phenol red and negligible riboflavin.
As i said I am relatively new at this, i possibly sound a bit naive - but we all have to start somewhere yes? anyway thanks for reading, if its any help im always looking for people to collaborate with - I work for public health england, and we have extensive facilities on site - please feel free to contact me if i can be of any assistance.
Best wishes,
Gareth.