Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Changes in the Salivary Metabolic Profile of Generalized Periodontitis Patients after Non-Surgical Periodontal Therapy. A Metabolomic Analysis Using Nuclear Magnetic Resonance Spectroscopy

Filippo Citterio
* ,
Federica Romano
ORCID logo ,
Gaia Meoni
ORCID logo ,
Giovanni Iaderosa
,
Silvia Grossi
,
Alberto Sobrero
,
Francesca Dego
,
Matteo Corana
,
Giovanni Nicolao Berta
ORCID logo ,
Leonardo Tenori
ORCID logo ,
Mario Aimetti
Version 1 : Received: 29 October 2020 / Approved: 30 October 2020 / Online: 30 October 2020 (13:28:58 CET)

A peer-reviewed article of this Preprint also exists.

Citterio, F.; Romano, F.; Meoni, G.; Iaderosa, G.; Grossi, S.; Sobrero, A.; Dego, F.; Corana, M.; Berta, G.N.; Tenori, L.; Aimetti, M. Changes in the Salivary Metabolic Profile of Generalized Periodontitis Patients after Non-surgical Periodontal Therapy: A Metabolomic Analysis Using Nuclear Magnetic Resonance Spectroscopy. J. Clin. Med. 2020, 9, 3977. Citterio, F.; Romano, F.; Meoni, G.; Iaderosa, G.; Grossi, S.; Sobrero, A.; Dego, F.; Corana, M.; Berta, G.N.; Tenori, L.; Aimetti, M. Changes in the Salivary Metabolic Profile of Generalized Periodontitis Patients after Non-surgical Periodontal Therapy: A Metabolomic Analysis Using Nuclear Magnetic Resonance Spectroscopy. J. Clin. Med. 2020, 9, 3977.

Abstract

Pattern analysis of salivary metabolic profile has been proven accurate to discriminate generalized periodontitis (GP) patients from healthy individuals (HI) as disease modifies the salivary concentrations of specific metabolites. Due to the scarcity of data in the literature, the aim of this study was to determine whether non-surgical periodontal therapy (NST) could change salivary metabolomic profile in GP to one more similar to HI. Unstimulated whole saliva of 11 HI and 12 GP patients were obtained prior to and 3 months after NST. Metabolic profiling was performed using Nuclear Magnetic Resonance (NMR) spectroscopy, followed by supervised multivariate statistical approach on entire saliva spectra and partial least square (PLS) discriminant analysis. In GP group, periodontal treatment improved all clinical parameters, but not all the diseased sites were eradicated. PLS revealed an accuracy of 100% in discriminating the metabolomic profile of each GP patient before and after NST. OPLS was able to discriminate the 3 groups of subjects with an accuracy of 85.6%. However the post-NST metabolic profile of GP patients could not be completely assimilated to that of HS. Although NST may produce significant changes in the metabolic profile, GP patients maintained a distinctive fingerprint compared to HI.

Keywords

diagnosis; metabolomics; periodontal therapy; periodontitis; saliva

Subject

Medicine and Pharmacology, Immunology and Allergy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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