How to cite:
Ramzan, M. A.; Asghar, H.; Rehman, A.; Rashid, M.; Jankuloski, L. In-silico Characterization and Comparative Analysis of BLB Disease Resistance Xa genes in Oryza sativa. Preprints2020, 2020100472. https://doi.org/10.20944/preprints202010.0472.v1
Ramzan, M. A.; Asghar, H.; Rehman, A.; Rashid, M.; Jankuloski, L. In-silico Characterization and Comparative Analysis of BLB Disease Resistance Xa genes in Oryza sativa. Preprints 2020, 2020100472. https://doi.org/10.20944/preprints202010.0472.v1
Ramzan, M. A.; Asghar, H.; Rehman, A.; Rashid, M.; Jankuloski, L. In-silico Characterization and Comparative Analysis of BLB Disease Resistance Xa genes in Oryza sativa. Preprints2020, 2020100472. https://doi.org/10.20944/preprints202010.0472.v1
APA Style
Ramzan, M. A., Asghar, H., Rehman, A., Rashid, M., & Jankuloski, L. (2020). <em>In-silico</em> Characterization and Comparative Analysis of BLB Disease Resistance Xa genes in <em>Oryza sativa</em>. Preprints. https://doi.org/10.20944/preprints202010.0472.v1
Chicago/Turabian Style
Ramzan, M. A., Muhammad Rashid and Ljupcho Jankuloski. 2020 "<em>In-silico</em> Characterization and Comparative Analysis of BLB Disease Resistance Xa genes in <em>Oryza sativa</em>" Preprints. https://doi.org/10.20944/preprints202010.0472.v1
Abstract
Globally, rice is utilized as staple food, belongs to the family Poaceae. From the past few decades under variable climate conditions, it greatly affected by the bacterial leaf blight (BLB) disease caused by a bacterium Xanthomonas oryzae pv. oryzae (Xoo). Scientists studied causes of the disease and found more than 61 isolates of Xoo. About 39 Xa genes were noted that cause race specifically resistance either individually or in pairs against Xoo. The canvas on the characterization of these genes is still unrevealed. In this study, amino acid sequences of Xa15, Xa19, Xa20 and Xa21 were mined and used for motif-domain identification, characterization, and comparative analysis. It includes screening of physical and chemical characteristics, sequence comparison to find sequence similarity between them and their phylogenetic comparison with other Xa genes and other species based upon LRR and S_TKc domains to find evolutionary relationship among them. The comparison-based modeling was performed and assessed by different tools to gain better understanding and structural evaluation. The results showed identified domains are specific in function, each domain involved in resistance against biotic and abiotic stresses through regulating different cellular processes. This study also revealed high similarity (>98% sequence identity) between these genes and encode a similar leucine-rich repeat receptor kinase like protein. It will optimize the breeding programs as it will be useful for the selection of effective genes to produce resistance in rice varieties against the specific strains of Xoo that will be more effective against BLB than the other Xa genes.
Keywords
BLB Resistance; Xa genes; Xoo; Molecular characterization; Rice; LRR; S_TKc
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
