Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Nootropic Effect of Fenugreek Seed Extract against Scopolamine Induced Cognitive Decline in Experimental Mice

Version 1 : Received: 26 September 2020 / Approved: 27 September 2020 / Online: 27 September 2020 (10:45:51 CEST)

How to cite: Mohamed Hussain, S.; Almutairi, N.; Alrakaf, F.; Aljameli, M.; Alshammari, M.; Alnasser, S. Nootropic Effect of Fenugreek Seed Extract against Scopolamine Induced Cognitive Decline in Experimental Mice. Preprints 2020, 2020090675 (doi: 10.20944/preprints202009.0675.v1). Mohamed Hussain, S.; Almutairi, N.; Alrakaf, F.; Aljameli, M.; Alshammari, M.; Alnasser, S. Nootropic Effect of Fenugreek Seed Extract against Scopolamine Induced Cognitive Decline in Experimental Mice. Preprints 2020, 2020090675 (doi: 10.20944/preprints202009.0675.v1).

Abstract

Background: Alzheimer’s disease affecting about 24 million people world-wide. The socio-economic burden on world-economies costing more than 172 billion US $ annually for the US alone. Objectives: To prepare aqueous extract of T. foenum graecum seeds (FSE) to explore the possible treatment for cognitive deficit in experimental animals. Materials and methods: FSE was subjected to preliminary phytochemical evaluation and antioxidant effect using free radical scavenging method (DPPH). All the animal behavior was video recorded with no human intervention during observation and animal groupings were blinded to avoid investigator bias. Different doses of FSE (5%, 10% and 20%), control, standard (Piracetam, 200 mg/kg, IP.) were given for male albino mice a period of 15 days followed by cognitive assessment in elevated plus maze and novel objection recognition tests. Ttransfer latencies and time exploring novel and familiar objects were recorded in respective tests. Retention of this learned-task was examined again 24 h later and inflexion ratio (IR) and discriminative index (DI) were calculated respectively. Next in the second set of experiment same groups and treatments were continued but scopolamine was administered to all the groups except normal control one hour after the last dose and examined similarly. Results: FSE showed potential antioxidant effect and a dose dependent increase in transfer latency and improved DI indicating a nootropic effect. FSE at 20% showed significant reversal of scopolamine induced dementia in the second set of experiment. Conclusion: FSE improved memory as well as reversed the chemically induced memory deficits in experimental mice.

Subject Areas

Fenugreek; Alzheimer’s disease; nootropic; cognitive disorders; herbs; memory

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