Torell, F.; Skotare, T.; Trygg, J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites2020, 10, 295.
Torell, F.; Skotare, T.; Trygg, J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites 2020, 10, 295.
Torell, F.; Skotare, T.; Trygg, J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites2020, 10, 295.
Torell, F.; Skotare, T.; Trygg, J. Application of Multiblock Analysis on Small Metabolomic Multi-Tissue Dataset. Metabolites 2020, 10, 295.
Abstract
Data integration has been proven to provide valuable information. The information extracted using data integration in the form of multiblock analysis can pinpoint both common and unique trends in the different blocks. When working with small multiblock datasets the number of possible integration methods is drastically reduced. To investigate the application of multiblock analysis in cases where one has few number of samples, we studied a small metabolomic multiblock dataset containing six blocks (i.e. tissue types), only including common metabolites. We used a single model multiblock analysis method called Joint and unique multiblock analysis (JUMBA) and compare it to a commonly used method, concatenated PCA. These methods were used to detect trends in the dataset and identify underlying factors responsible for metabolic variations. Using JUMBA, we were able to interpret the extracted components and link them to relevant biological properties. JUMBA shows how the observations are related to one another, the stability of these relationships and to what extent each of the blocks contribute to the components. These results indicate that multiblock methods can be useful even with a small number of samples.
Keywords
Data integration; Metabolomics; Multi-tissue; Multiblock; Joint and unique multiblock analysis (JUMBA), OnPLS; Multiblock Orthogonal Component Analysis (MOCA)
Subject
Biology and Life Sciences, Endocrinology and Metabolism
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
