Preprint Article Version 1 This version is not peer-reviewed

Synergistic Hepatoprotective Interaction between α-Tocopherol and Ascorbic Acid on Paracetamol Induced Liver Damage in Rats

Version 1 : Received: 8 June 2020 / Approved: 9 June 2020 / Online: 9 June 2020 (11:40:38 CEST)

How to cite: Khan, S.I.; Begum, M.; Chowdhury, R.; Rahman, M.M.; Asaduzzaman, M. Synergistic Hepatoprotective Interaction between α-Tocopherol and Ascorbic Acid on Paracetamol Induced Liver Damage in Rats. Preprints 2020, 2020060122 (doi: 10.20944/preprints202006.0122.v1). Khan, S.I.; Begum, M.; Chowdhury, R.; Rahman, M.M.; Asaduzzaman, M. Synergistic Hepatoprotective Interaction between α-Tocopherol and Ascorbic Acid on Paracetamol Induced Liver Damage in Rats. Preprints 2020, 2020060122 (doi: 10.20944/preprints202006.0122.v1).

Abstract

Background and objectives: The hepatoprotective activity of vitamin E and C is evident due to their ability of modulating the antioxidant pathway. In this study, we have evaluated the effects of α-tocopherol and ascorbic acid on paracetamol induced liver damage with offsetting various levels of drug treatment following an in vivo experimental protocol on Wistar albino male rats. Materials and Methods: The level of lipid peroxidation as well as histological examination of liver tissues were observed among 50 Wistar albino male rats to evaluate hepatoprotective effect of α-tocopherol and ascorbic acid on hepatocytes. The experiment was divided into 5 groups (10 rats in each group)- Basal control group (Group-I, with propylene glycol), Paracetamol treated control group (Group –II), α-tocopherol pretreated & paracetamol treated group (Group –III), Ascorbic acid pretreated & paracetamol treated group (Group –IV) and Ascorbic acid pretreated & paracetamol treated group (Group –IV). Results: The mean (± SD) Malondialdehyde (MDA) concentration were significantly reduced in α-tocopherol pretreated and paracetamol treated group (P<0.001), Ascorbic acid pretreated and paracetamol treated group (P≤0.05) and combined α-tocopherol with ascorbic acid pretreated & paracetamol treated group (P<0.001). Statistically significant differences in histological findings of rat liver were observed in paracetamol treated control group (P<0.001), ascorbic acid pretreated and paracetamol treated group (P<0.001). The serum alanine aminotransferase (ALT) level was also significantly higher in paracetamol treated group (P<0.001), α-tocopherol pretreated plus paracetamol treated group (P≤0.05) and in ascorbic acid pretreated plus paracetamol treated group (P<0.001). Conclusion: The combined pretreatment of α-tocopherol & ascorbic acid have better hepatoprotective effects than α-tocopherol or ascorbic acid alone against paracetamol induced liver damage. The decrement of free radicals produced by vitamin E could be a better hepatoprotective antioxidant than vitamin C in paracetamol induced toxicity.

Subject Areas

Hepatoprotective; ascorbic acid; α-tocopherol; paracetamol; ALT; MDA; Histology

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