Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Differentially-Charged Phospholipids Interact with Alphaherpesviruses and Interfere with Virus Entry

Version 1 : Received: 13 April 2020 / Approved: 14 April 2020 / Online: 14 April 2020 (08:54:01 CEST)

How to cite: Kolyvushko, O.; Latzke, J.; Dahmani, I.; Osterrieder, N.; Chiantia, S.; Azab, W. Differentially-Charged Phospholipids Interact with Alphaherpesviruses and Interfere with Virus Entry. Preprints 2020, 2020040220 (doi: 10.20944/preprints202004.0220.v1). Kolyvushko, O.; Latzke, J.; Dahmani, I.; Osterrieder, N.; Chiantia, S.; Azab, W. Differentially-Charged Phospholipids Interact with Alphaherpesviruses and Interfere with Virus Entry. Preprints 2020, 2020040220 (doi: 10.20944/preprints202004.0220.v1).

Abstract

Exposure of phosphatidylserine (PS) in the outer leaflet of the plasma membrane is induced by infection with several members of the Alphaherpesvirinae subfamily. There is evidence that PS is used by the equine herpesvirus type 1 (EHV-1) during entry, but the exact role of PS and other phospholipids in the entry process remains unknown. Here, we investigated the interaction of differently charged phospholipids with virus particles and determined their influence on infection. Our data show that liposomes containing negatively charged PS or positively charged DOTAP [N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium)] inhibited EHV-1 infection, while neutral phosphatidylcholine (PC) had no effect. Inhibition of infection with PS was transient, decreased with time, and was dose dependent. Our findings indicate that both cationic and anionic phospholipids can interact with the virus and reduce infectivity, while acting through different mechanisms. Charged phospholipids were found to have antiviral effects and can may be used to inhibit EHV-1 infection.

Subject Areas

alphaherpesvirus; EHV-1; phosphatidylserine; inhibition; pathogen host interaction

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.