Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of in ovo Supplementation with Nano-nutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

Sivakumar Subramaniyan
,
Kang Darae
ORCID logo ,
Sharif Hasan Siddiqui
,
Jin Ryong Park
,
Weishun Tian
,
Yong Park Byung
,
Kwan Seob Shim
*
Version 1 : Received: 4 March 2020 / Approved: 4 March 2020 / Online: 4 March 2020 (10:45:11 CET)

A peer-reviewed article of this Preprint also exists.

Subramaniyan, S.A.; Kang, D.; Siddiqui, S.H.; Park, J.; Tian, W.; Park, B.; Shim, K. Effects of In Ovo Supplementation with Nanonutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages. Animals 2020, 10, 564. Subramaniyan, S.A.; Kang, D.; Siddiqui, S.H.; Park, J.; Tian, W.; Park, B.; Shim, K. Effects of In Ovo Supplementation with Nanonutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages. Animals 2020, 10, 564.

Abstract

The aim of the study was to analyze the in ovo injection of chemically and biologically synthesized silver nano-particles (Ag NPs) using Brassica oleracea L. var capitate f. Rubra, (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of the broiler chickens. L-Arg (100 μg) conjugated with 1000 µg of Ag NPs synthesized by (BOL)-extract and L-Arg (100 μg) conjugated with 100 µg of Ag NPs chemically synthesized were injected into fertile eggs at 8d, 14d and 18d of incubation. Living embryo and hatched chicks were calculated. Survivability and hatchability were not affected by the injected dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) but it significantly improved when the eggs were injected on day 14 of incubation compared with those injected on days 8 or 18. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly up-related in 14 d of incubation whereas the heat shock proteins (HSPs) such as HSP-60 and HSP-70 were significantly up-regulated in 18 d incubation. In addition, the in ovo injection on 18 d significantly increased the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) as well the immunoglobulin (IgM) levels were increased in 14d incubation period in serum at the same concentration.

Keywords

nanoparticles; silver; L-arginine; conjugation; muscle growth

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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