Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Version 1 : Received: 5 November 2019 / Approved: 5 November 2019 / Online: 5 November 2019 (03:12:59 CET)

How to cite: Yoshida, K.; Kashimura, Y.; Kamijo, T.; Ono, T.; Dairaku, T.; Sato, T.; Kashiwagi, Y.; Sato, K. Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase. Preprints 2019, 2019110043. https://doi.org/10.20944/preprints201911.0043.v1 Yoshida, K.; Kashimura, Y.; Kamijo, T.; Ono, T.; Dairaku, T.; Sato, T.; Kashiwagi, Y.; Sato, K. Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase. Preprints 2019, 2019110043. https://doi.org/10.20944/preprints201911.0043.v1

Abstract

Glucose-sensitive films were prepared by the layer-by-layer (LbL) deposition of poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA+GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA+GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve a more reactive oxygen species (ROS) that was formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the H-PEI/DNA+GOx)5 film was observed when the thin film was immersed in a glucose solution. A (H-PEI/DNA+GOx)5 film exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated decomposition of the film increased with the time. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strength close to physiological conditions, it was possible to slowly decompose the LbL film at a sub-millimolar glucose concentration.

Keywords

hydrogen peroxide response; layer-by-layer; multilayer thin film; glucose sensitive; stimuli-sensitive

Subject

Chemistry and Materials Science, Medicinal Chemistry

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.