Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Protective Role of Toll-like Receptors Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis

Ibrahim Aly
,
Essam H. Ibrahim
* ORCID logo ,
Rabab S. Hamad
,
Hoda EL Sayed
,
Sama M.N. Attiyah
,
Wafaa E-Komy
,
Hamed A. Ghramh
,
Ali Alshehri
,
Khalid M. Alsyaad
,
Mohammed Alshehri
,
Mona Kilany
Version 1 : Received: 14 September 2019 / Approved: 16 September 2019 / Online: 16 September 2019 (01:27:58 CEST)

How to cite: Aly, I.; Ibrahim, E.H.; Hamad, R.S.; Sayed, H.E.; Attiyah, S.M.; E-Komy, W.; Ghramh, H.A.; Alshehri, A.; Alsyaad, K.M.; Alshehri, M.; Kilany, M. The Protective Role of Toll-like Receptors Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis. Preprints 2019, 2019090157. https://doi.org/10.20944/preprints201909.0157.v1 Aly, I.; Ibrahim, E.H.; Hamad, R.S.; Sayed, H.E.; Attiyah, S.M.; E-Komy, W.; Ghramh, H.A.; Alshehri, A.; Alsyaad, K.M.; Alshehri, M.; Kilany, M. The Protective Role of Toll-like Receptors Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis. Preprints 2019, 2019090157. https://doi.org/10.20944/preprints201909.0157.v1

Abstract

Schistosomiasis, a crippling ailment that afflicts over 220 million people worldwide. Yet or up till now, there is no vaccine for schistosomiasis, and chemotherapy relies heavily on a single drug, the praziquantel. The present study was undertaken to investigate the therapeutic effect of Monophosphoryl Lipid A (MPLA) as an adjuvant in soluble egg antigen (SEA) vaccinated mice against the deleterious pathological impacts induced in hepatic tissues of mice by Schistosoma mansoni infection. In addition, to study the associated parasitological, immunological and biochemical parameters. Parasitological parameters showed that intraperitoneal injection of MPLA into SEA-vaccinated and S. mansoni-infected mice was effective to a significant degree in reducing the worm and egg burden, granuloma count and diameter as well as the total area of infection in their livers versus SEA-untreated but infected ones. In addition, MPLA showed ameliorative action on the elevated liver oxidative stress marker, including malondialdehyde (MDA) and decrease in the level of the antioxidant enzymes, reduced glutathione (GSH) and catalase (CAT) which may have a role in the liver damage and fibrosis due to S. mansoni infection. In conclusion, treatment with MPLA has multi-functions in attenuating the deleterious impacts of S. mansoni infection in mice livers. Its effects are mediated through a reduction of ova count, worm burden, granuloma diameter and amelioration of antioxidant defense systems, and liver function biomarkers.

Keywords

schistosomiasis; monophosphoryl lipid A (MPLA); chemotherapy; oxidative stress; antioxidant enzymes; SEA

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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