Preprint Article Version 1 This version is not peer-reviewed

Treatment with DHA Improves Epidermal Eeratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models

Version 1 : Received: 15 August 2019 / Approved: 16 August 2019 / Online: 16 August 2019 (08:17:51 CEST)

A peer-reviewed article of this Preprint also exists.

Jia, T.; Qiao, W.; Yao, Q.; Wu, W.; Kaku, K. Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models. Molecules 2019, 24, 3156. Jia, T.; Qiao, W.; Yao, Q.; Wu, W.; Kaku, K. Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models. Molecules 2019, 24, 3156.

Journal reference: Molecules 2019, 24, 3156
DOI: 10.3390/molecules24173156

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease, which can cause skin barrier function damaged. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect in deficient skin model, there is no study to investigate the effects of topical treatment with DHA in inflammatory reconstructed human epidermis (RHE) model. The effects of DHA on monolayer normal human epidermal keratinocyte (NHEK) cells were evaluated via CCK-8, qPCR and ELISA. The skin related barrier function was assessed by hematoxylin-eosin (HE) staining, western blot (WB), Immunohistofluorescence (IF) and ELISA in normal and inflammatory RHE models. DHA upregulated filaggrin and loricrin expression at mRNA levels in addition to suppress overexpression of TNF-α,IL-1α and IL-6 stimulated by poly I:C plus LPS (stimulation cocktail) in cultured NHEK cells. After topical treatment with DHA, cocktail induced inflammatory characteristics of skin diseases including barrier morphological, differentiation proteins and TSLP secretion, which were alleviated in RHE models. Supplementation with DHA can improved related barrier function and have anti-inflammation effects in monolayer keratinocytes and RHE models, which indicated that DHA may have a potential value for the treatment of inflammation-associate skin diseases.

Subject Areas

DHA; reconstructed human models; filaggrin; skin barrier; inflammation

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