Preprint Article Version 1 This version is not peer-reviewed

Tissue-Engineered Anterior Segment Eye Cultures Display an Intraocular Pressure Homeostatic Response

Version 1 : Received: 8 July 2019 / Approved: 10 July 2019 / Online: 10 July 2019 (09:53:49 CEST)

How to cite: Waxman, S.; Wang, C.; Dang, Y.; Loewen, R.; Loewen, N.A. Tissue-Engineered Anterior Segment Eye Cultures Display an Intraocular Pressure Homeostatic Response. Preprints 2019, 2019070142 (doi: 10.20944/preprints201907.0142.v1). Waxman, S.; Wang, C.; Dang, Y.; Loewen, R.; Loewen, N.A. Tissue-Engineered Anterior Segment Eye Cultures Display an Intraocular Pressure Homeostatic Response. Preprints 2019, 2019070142 (doi: 10.20944/preprints201907.0142.v1).

Abstract

Glaucoma is a blinding disease largely caused by increased resistance to drainage of fluid from the eye's anterior chamber, resulting in elevated intraocular pressure (IOP). A major site of fluid outflow regulation and pathology is the trabecular meshwork (TM), an extracellular matrix (ECM)-rich tissue at the entrance of the eye's drainage system. We aimed to characterize the structural and functional properties of a newly developed tissue-engineered (TE) anterior segment eye culture model. We hypothesized that repopulation of decellularized TM ECM with non-native TM cells could restore intraocular pressure (IOP) homeostatic ability. Decellularized porcine anterior segment scaffolds demonstrated complete removal of cells, significant reduction of DNA content, and well-preserved ECM ultrastructure. Seeded cells localized to the TM region (p < 0.001) and progressively infiltrated meshwork ECM. Cells reached a distribution comparable to control TM after four days of perfusion culture. After perfusion rate increase challenge, TE cultures maintained healthy IOPs through regulation of outflow resistance (reseeded = 16.53 ± 0.89, decellularized = 35.23 ± 2.20 mmHg, p < 0.0001). In conclusion, we describe a readily available, storable, and biocompatible scaffold for anterior segment perfusion culture of non-native cells. TE organs demonstrated physiological similarities to native tissues and may reduce the need for scarce donor globes in outflow research.

Subject Areas

glaucoma; tissue engineering; trabecular meshwork; outflow facility

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.