Preprint Article Version 1 This version is not peer-reviewed

The Brain Origin of Neonatal Microglia Determines the Content of Exosomes and Biological Function

Version 1 : Received: 6 July 2019 / Approved: 8 July 2019 / Online: 8 July 2019 (04:26:49 CEST)

How to cite: Murgoci, A.; Mallah, K.; Aboulouard, S.; Lefebvre, C.; Cizek, M.; Fournier, I.; Cizkova, D.; Salzet, M. The Brain Origin of Neonatal Microglia Determines the Content of Exosomes and Biological Function. Preprints 2019, 2019070102 (doi: 10.20944/preprints201907.0102.v1). Murgoci, A.; Mallah, K.; Aboulouard, S.; Lefebvre, C.; Cizek, M.; Fournier, I.; Cizkova, D.; Salzet, M. The Brain Origin of Neonatal Microglia Determines the Content of Exosomes and Biological Function. Preprints 2019, 2019070102 (doi: 10.20944/preprints201907.0102.v1).

Abstract

Using a combination of pan proteomic platform associated with systemic biology analyses, we demonstrate that neonatal microglial cells derived from cortex and spinal cord expressed different phenotypes upon the physiological or pathological conditions. They also highlight great variability in protein production on both cellular and exosome levels. Bioinformatics data indicate for the cortical microglia anti-inflammatory and neurogenesis/tumorigenesis characteristics, while for the spinal cord microglia involvement in the inflammatory response. We confirmed these results by performing functional testing including neurite outgrowth assays in DRGs cell line, and glioma proliferation analysis in 3D spheroid cultures. Results from these in vitro assays indicate that the microglia located at different CNS areas reveal differential biological functions. While both microglia sources enhanced growth of DRGs axons, only the spinal microglia significantly attenuated glioma proliferation. Overall these findings are pointing to the fact that the origin of neonatal microglia affects the physio-pathological function, which may address the prevalence of the glioma in the brain in comparison with the spinal cord in adult.

Subject Areas

microglia exosomes; 3D culture; proteomic study; glioma; neurites outgrowth

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