Preprint Technical Note Version 1 This version is not peer-reviewed

Mapping Quantitative Trait Loci onto Chromosome-scale Pseudomolecules in Flax

Version 1 : Received: 10 January 2019 / Approved: 14 January 2019 / Online: 14 January 2019 (07:19:08 CET)

How to cite: You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-scale Pseudomolecules in Flax. Preprints 2019, 2019010126 (doi: 10.20944/preprints201901.0126.v1). You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-scale Pseudomolecules in Flax. Preprints 2019, 2019010126 (doi: 10.20944/preprints201901.0126.v1).

Abstract

Quantitative trait loci (QTL) are genomic regions associated with phenotype variation of quantitative traits in a population. To date, a total of 267 QTL for 29 quantitative traits have been reported in 13 studies on flax. Of these, 200 QTL from 12 studies were identified based on genetic maps, scaffold sequences, or pre-released chromosome-scale pseudomolecules. Molecular markers for QTL identification differed across studies but were mainly based on simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers. This article provides methods with software tools and database files to uniquely map SSR and SNP markers from different references onto the recently released chromosome-scale pseudomolecules. Using these methods, 195 QTL were successfully sorted onto the 15 flax chromosomes and grouped into 133 co-located QTL clusters. Mapping of QTL from different studies to the same reference enables comparisons and facilitates genome-wide QTL analysis, candidate gene scanning, and breeding applications.

Subject Areas

flax; association mapping; genome-wide association study (GWAS); simple sequence repeat (SSR); single nucleotide polymorphism (SNP); quantitative trait loci (QTL); chromosome-scale pseudomolecules

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