You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax. Methods and Protocols 2020, 3, 28, doi:10.3390/mps3020028.
You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax. Methods and Protocols 2020, 3, 28, doi:10.3390/mps3020028.
You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax. Methods and Protocols 2020, 3, 28, doi:10.3390/mps3020028.
You, F.M.; Cloutier, S. Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax. Methods and Protocols 2020, 3, 28, doi:10.3390/mps3020028.
Abstract
Quantitative trait loci (QTL) are genomic regions associated with phenotype variation of quantitative traits in a population. To date, a total of 267 QTL for 29 quantitative traits have been reported in 13 studies on flax. Of these, 200 QTL from 12 studies were identified based on genetic maps, scaffold sequences, or pre-released chromosome-scale pseudomolecules. Molecular markers for QTL identification differed across studies but were mainly based on simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers. This article provides methods with software tools and database files to uniquely map SSR and SNP markers from different references onto the recently released chromosome-scale pseudomolecules. Using these methods, 195 QTL were successfully sorted onto the 15 flax chromosomes and grouped into 133 co-located QTL clusters. Mapping of QTL from different studies to the same reference enables comparisons and facilitates genome-wide QTL analysis, candidate gene scanning, and breeding applications.
Keywords
flax; association mapping; genome-wide association study (GWAS); simple sequence repeat (SSR); single nucleotide polymorphism (SNP); quantitative trait loci (QTL); chromosome-scale pseudomolecules
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
