Amado, D.; Velez, N.; Ceballos-Garzon, A.; Monroy, J.; Parra-Giraldo, C.-M. Uses and Advantages of CRISPR/Cas Genetic Edition in Yeasts. Preprints2018, 2018120001. https://doi.org/10.20944/preprints201812.0001.v1
APA Style
Amado, D., Velez, N., Ceballos-Garzon, A., Monroy, J., & Parra-Giraldo, C. M. (2018). Uses and Advantages of CRISPR/Cas Genetic Edition in Yeasts. Preprints. https://doi.org/10.20944/preprints201812.0001.v1
Chicago/Turabian Style
Amado, D., Juan Monroy and Claudia-Marcela Parra-Giraldo. 2018 "Uses and Advantages of CRISPR/Cas Genetic Edition in Yeasts" Preprints. https://doi.org/10.20944/preprints201812.0001.v1
Abstract
This review summarizes the use of CRISPR system in yeasts, identifying advantages and disadvantages of its applications. 39 articles were evaluated including 12 articles that discussed the advantages of new CRISPR systems that improved the initial system, and another 27 were evaluated, among these: three were applications in Cryptococcus neoformans, four in candida sp., three in Schizosaccharomyces pombe, nine in Saccharomyces cerevisiae, four in Yarrowia lipolytica, and four in industrially important yeasts such as Pichia pastoris and Saccharomyces pastorianus. It was concluded that the CRISPR system is one of the most versatile genetic editing systems available nowadays. It can be applied in different organisms for several effects including gene knock-outs, performing point mutations, gene expression, or even applying multiple edition operations in several genes. However, we recognize that numerous studies lack a control group of the mutated strains, which leaves many questions unanswered. For instance, the extent and precision of this techniques, it also represents a risk to biosecurity standards. Therefore, this review shows the compilation of CRISPR system information, which could be used to generate different alternatives in the industry and clinical fields.
Keywords
CRISPR system; yeast; gene edition
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
