Preprint Review Version 1 This version is not peer-reviewed

Monitoring HIV-1 Assembly in Living Cells: Insight from Dynamic and Single Molecule Microscopy

Version 1 : Received: 23 November 2018 / Approved: 26 November 2018 / Online: 26 November 2018 (14:12:08 CET)

A peer-reviewed article of this Preprint also exists.

Inamdar, K.; Floderer, C.; Favard, C.; Muriaux, D. Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy. Viruses 2019, 11, 72. Inamdar, K.; Floderer, C.; Favard, C.; Muriaux, D. Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy. Viruses 2019, 11, 72.

Journal reference: Viruses 2019, 11, 72
DOI: 10.3390/v11010072

Abstract

HIV-1 assembly is a complex mechanism taking place at the plasma membrane of the host cell. It requires nice spatial and temporal coordination to end up with a full immature virus. Researchers have extensively studied HIV-1 assembly molecular mechanism during the past decades, in order to dissect the respective roles of viral proteins, viral genome and host cell factors. Nevertheless, the time course of the process has been observed in living cells only a decade ago. The very recent revolution of optical microscopy, combining high speed and high spatial resolution now permit to study assemblies and their consequences at the single molecule level within (living) cells. In this review, after a short description of these new approaches, we will show how HIV-1 assembly in cells has been revisited using these advanced super resolution microscopy techniques and how much it could make a bridge in studying assembly from the single molecule to the host cell.

Subject Areas

HIV-1 assembly, Gag, single molecule microscopy, dynamics

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