Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Aptamers Selected for Recognizing Amyloid β-protein—A Case for Cautious Optimism

Version 1 : Received: 5 February 2018 / Approved: 5 February 2018 / Online: 5 February 2018 (22:37:02 CET)

A peer-reviewed article of this Preprint also exists.

Rahimi, F. Aptamers Selected for Recognizing Amyloid β-Protein—A Case for Cautious Optimism. Int. J. Mol. Sci. 2018, 19, 668. Rahimi, F. Aptamers Selected for Recognizing Amyloid β-Protein—A Case for Cautious Optimism. Int. J. Mol. Sci. 2018, 19, 668.


Aptamers are versatile oligonucleotide ligands used for molecular recognition of diverse targets. However, application of aptamers to the field of amyloid β-protein (Aβ) has been limited so far. Aβ is an intrinsically disordered protein that exists in a dynamic conformational equilibrium, presenting time-dependent ensembles of short-lived, metastable structures and assemblies that have been generally difficult to isolate and characterize. Moreover, despite understanding of potential physiological roles of Aβ, this peptide has been linked to the pathogenesis of Alzheimer disease, and its pathogenic roles remain controversial. Accumulated scientific evidence thus far highlights undesirable or nonspecific interactions between selected aptamers and different Aβ assemblies likely due to metastable nature of Aβ or inherent affinity of RNA oligonucleotides to β-sheet-rich fibrillar structures of amyloidogenic proteins. Accordingly, lessons drawn from Aβ–aptamer studies emphasize that purity and uniformity of the protein target and rigorous characterization of aptamers’ specificity are important for realizing and garnering the full potential of aptamers selected for recongizing Aβ or other intrinsically disordered proteins. This review summarizes studies of aptamers selected for recognizing different Aβ assemblies and highlights controversies, difficulties, and limitations of such studies.


Alzheimer disease; Amyloid β-protein; Antibodies; Cross-reactions; Nucleotide aptamers; Oligonucleotide ligands; Systematic evolution of ligands by exponential enrichment; Specificity; Therapeutics


Biology and Life Sciences, Biochemistry and Molecular Biology

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