Preprint Article Version 1 This version is not peer-reviewed

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

Version 1 : Received: 13 January 2017 / Approved: 13 January 2017 / Online: 13 January 2017 (13:17:13 CET)

A peer-reviewed article of this Preprint also exists.

Gasmi, L.; Ferré, J.; Herrero, S. High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae). Biosensors 2017, 7, 12. Gasmi, L.; Ferré, J.; Herrero, S. High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae). Biosensors 2017, 7, 12.

Journal reference: Biosensors 2017, 7, 12
DOI: 10.3390/bios7010012

Abstract

Lectins are carbohydrate-interacting proteins playing a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through the carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensor for capturing and identification of bacterial species. In this work, we have characterized the bacterial agglutination properties of three C-type lectins from the Lepidoptera Spodoptera exigua. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as biosensor or other biotechnological applications involving bacterial capturing.

Subject Areas

C-type lectin; agglutination; CRD; bacterial detection; E. coli

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