Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Influence of fruit load and water deficit on olive fruit phenolic profiling and yield

Version 1 : Received: 19 April 2024 / Approved: 22 April 2024 / Online: 22 April 2024 (18:41:09 CEST)

How to cite: Farolfi, C.; Tombesi, S.; Lucini, L.; Capri, E.; García-Pérez, P. Influence of fruit load and water deficit on olive fruit phenolic profiling and yield. Preprints 2024, 2024041440. https://doi.org/10.20944/preprints202404.1440.v1 Farolfi, C.; Tombesi, S.; Lucini, L.; Capri, E.; García-Pérez, P. Influence of fruit load and water deficit on olive fruit phenolic profiling and yield. Preprints 2024, 2024041440. https://doi.org/10.20944/preprints202404.1440.v1

Abstract

High-density olive groves, despite their interesting production potential, have several limitations, also due to the high fruit load and the irrigation requirements.The study aimed to evaluate the effects of fruit load and deficit irrigation on oil yield and fruit quality, and on olive chemical composition in a high-density olive grove (cv Sikitita). Our main hypothesis was that the primary metabolism, as influenced by crop load and stress, could modify the accumulation of different phenolic classes.Different fruit loads were generated through flower thinning (66%, 50%, 33%, 0%) and two deficit irrigation treatments (-60%, -75%) were compared to the well-watered control (920 m3/ha).Thinning treatments had a limited effect on oil yield, on the other hand, deficit irrigation caused considerably less oil accumulation in the fruit on all sampling dates.Thinning 66% and deficit irrigation 75% were considered with the control for untargeted metabolomic analysis, considering three sampling dates. 233 different phenolic compounds were annotated. Multivariate HCA results indicated the impact of harvest time on the phenolic profile of olive fruits, yielding two separate clusters grouping t1 and t2 together and apart from t3. Regarding agronomic techniques, they played a differential role in the phenolic profile (supervised OPLS – DA). Fruit load mostly affected flavonoid glycosides. In contrast, the phenolic response to deficit irrigation is more heterogeneous, with phenolic acids (35%), flavonoids (25%), LMW, and other phenols (25%).

Keywords

crop load, deficit irrigation, metabolomics, secondary metabolites, phenolic compounds.

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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