Version 1
: Received: 17 April 2024 / Approved: 18 April 2024 / Online: 18 April 2024 (11:00:02 CEST)
How to cite:
Łach, Ł.; Khomutovska, N.; Kwiatowski, J.; Jasser, I. The selectivity of 16S primers influences the results of the diversity of cyanobacteria from small water bodies. Preprints2024, 2024041223. https://doi.org/10.20944/preprints202404.1223.v1
Łach, Ł.; Khomutovska, N.; Kwiatowski, J.; Jasser, I. The selectivity of 16S primers influences the results of the diversity of cyanobacteria from small water bodies. Preprints 2024, 2024041223. https://doi.org/10.20944/preprints202404.1223.v1
Łach, Ł.; Khomutovska, N.; Kwiatowski, J.; Jasser, I. The selectivity of 16S primers influences the results of the diversity of cyanobacteria from small water bodies. Preprints2024, 2024041223. https://doi.org/10.20944/preprints202404.1223.v1
APA Style
Łach, Ł., Khomutovska, N., Kwiatowski, J., & Jasser, I. (2024). The selectivity of 16S primers influences the results of the diversity of cyanobacteria from small water bodies. Preprints. https://doi.org/10.20944/preprints202404.1223.v1
Chicago/Turabian Style
Łach, Ł., Jan Kwiatowski and Iwona Jasser. 2024 "The selectivity of 16S primers influences the results of the diversity of cyanobacteria from small water bodies" Preprints. https://doi.org/10.20944/preprints202404.1223.v1
Abstract
The majority of investigations on microbial communities from various environments are presently built on culture-independent methods. Many studies point to the pivotal, selective role of primers targeting hypervariable regions of 16S rRNA in the metabarcoding of bacteria, including cyanobacterial communities. The selectivity of primers designed to amplify targeted regions of the 16S rRNA gene, which has been highlighted by many authors, limited effective amplification. Moreover, the type and specificity of the studied material can also negatively influence the results of 16S metabarcoding. Most of the studies of cyanobacterial communities have been performed for planktonic microbial communities that are often represented by common, well-studied species. In this study, we present the results of 16S metabarcoding analysis using three primer pairs - two already well-known and a third designed in this study - that amplify divergent regions of the 16S rRNA gene (V3-V4, V4-V6, V6) for benthic, microbial mat-forming cyanobacteria communities. Such communities can be a source of toxigenic cyanobacterial taxa and should be monitored with adequate primers. The comparison of three primer pairs suggested that those designed within the present study describe the structure and composition of highly heterogeneous cyanobacterial mats’ communities better than the others
Keywords
cyanobacteria; NGS; microbial mats; novel primer pairs V3-V4; novel primer pairs V4-V6; novel primer pairs V6
Subject
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
