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Optimization of the Conditions for the Transformation of a Bacillus subtilis Strain L11 to Prepare Nano Selenium and Its Preliminary Application in Sheep Feed
Guo, W.; Shi, X.; Wang, L.; Cong, X.; Cheng, S.; Li, L.; Cheng, H. Optimization of the Conditions for the Transformation of a Bacillus subtilis Strain L11 to Prepare Nano Selenium and Its Preliminary Application in Sheep Feed. Microbiol. Res.2024, 15, 326-341.
Guo, W.; Shi, X.; Wang, L.; Cong, X.; Cheng, S.; Li, L.; Cheng, H. Optimization of the Conditions for the Transformation of a Bacillus subtilis Strain L11 to Prepare Nano Selenium and Its Preliminary Application in Sheep Feed. Microbiol. Res. 2024, 15, 326-341.
Guo, W.; Shi, X.; Wang, L.; Cong, X.; Cheng, S.; Li, L.; Cheng, H. Optimization of the Conditions for the Transformation of a Bacillus subtilis Strain L11 to Prepare Nano Selenium and Its Preliminary Application in Sheep Feed. Microbiol. Res.2024, 15, 326-341.
Guo, W.; Shi, X.; Wang, L.; Cong, X.; Cheng, S.; Li, L.; Cheng, H. Optimization of the Conditions for the Transformation of a Bacillus subtilis Strain L11 to Prepare Nano Selenium and Its Preliminary Application in Sheep Feed. Microbiol. Res. 2024, 15, 326-341.
Abstract
Selenium nanoparticles (SeNPs) has higher bioavailability and safety than inorganic selenium, and was widely used in medical, agricultural, nutritional supplements, and antibacterial fields. The present study screened a strain L11 producing SeNPs from a selenium rich dairy cow breeding base in Hubei Province, China. The strain was identified as Bacillus subtilis through physiological, biochemical, and molecular biology analysis. Through optimization of cultivation conditions, the optimal cultivation parameters for L11 to produce SeNPs were pH=6, cultivation temperature of 37 °C, 4 mmol/L Na2SeO3, and cultivation for 48 hours. XPS, SEM-EDS, and TEM were used to verify that the Se particles produced by L11 are SeNPs with diameters ranging from 50 to 200 nm. The combination of protein analysis of different cell components and TEM analysis showed that L11 mainly produces SeNPs through the transformation of the cell's periplasmic space, cell membrane, and cell wall. Adding L11 SeNPs complex to sheep feed can significantly enhance the antioxidant activity and immunity of sheep, and increase the Se content in the neck muscles, liver, and spleen tissues.
Biology and Life Sciences, Agricultural Science and Agronomy
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