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Effects of Organic Selenium and Nano-Selenium on Drought Stress of Pak Choi (Brassica chinensis var. pekinensis. cv. ‘Suzhouqing’) and Its Transcriptomic Analysis
Wang, Y.; Rao, C.; Huang, L.; Wu, J.; Sun, P.; Zhan, J.; Wu, J.; Liu, S.; Zhou, C.; Hu, L.; Li, N.; Chen, J.; He, X. Effects of Organic Selenium and Nanoselenium on Drought Stress of Pak Choi (Brassica chinensis var. pekinensis. cv.‘Suzhouqing’) and Its Transcriptomic Analysis. Agronomy2024, 14, 78.
Wang, Y.; Rao, C.; Huang, L.; Wu, J.; Sun, P.; Zhan, J.; Wu, J.; Liu, S.; Zhou, C.; Hu, L.; Li, N.; Chen, J.; He, X. Effects of Organic Selenium and Nanoselenium on Drought Stress of Pak Choi (Brassica chinensis var. pekinensis. cv.‘Suzhouqing’) and Its Transcriptomic Analysis. Agronomy 2024, 14, 78.
Wang, Y.; Rao, C.; Huang, L.; Wu, J.; Sun, P.; Zhan, J.; Wu, J.; Liu, S.; Zhou, C.; Hu, L.; Li, N.; Chen, J.; He, X. Effects of Organic Selenium and Nanoselenium on Drought Stress of Pak Choi (Brassica chinensis var. pekinensis. cv.‘Suzhouqing’) and Its Transcriptomic Analysis. Agronomy2024, 14, 78.
Wang, Y.; Rao, C.; Huang, L.; Wu, J.; Sun, P.; Zhan, J.; Wu, J.; Liu, S.; Zhou, C.; Hu, L.; Li, N.; Chen, J.; He, X. Effects of Organic Selenium and Nanoselenium on Drought Stress of Pak Choi (Brassica chinensis var. pekinensis. cv.‘Suzhouqing’) and Its Transcriptomic Analysis. Agronomy 2024, 14, 78.
Abstract
In order toinvestigate both the physiological and molecular mechanisms underlying the impacts of organic selenium and nano-selenium on drought stress of Pak Choi (Brassica chinensis var. pekinensis. cv. ‘Suzhouqing’), we measured photosynthetic pigment, antioxidant, nutritional quality, and performed transcriptome sequencing of leaves using RNA-seq technology. Under drought conditions, the beneficial effects of organic selenium and nano-selenium on weight and quality of ‘Suzhouqing’ were primarily manifested in the following aspects. Firstly, there was an enhancement in photosynthetic capacity through upregulation of light-trapping pigment proteins: Lhca1, Lhca2, Lhca3, Lhca4, Lhcb1, Lhcb2, Lhcb3, Lhcb4 and Lhcb5. Secondly, effective regulation of reactive oxygen species homeostasis was achieved by activating the antioxidant system via up-regulation of glutathione S-transferase. Thirdly, water homeostasis was maintained through glutathione oxidase activity. Lastly, increased expression levels of ABC transporter, adenylate kinase and cysteine desulphurase contributed to elevated total selenium content. In addition, we screened hub core genes related to the above key metabolic pathways through Weighted Gene Co-expression Network Analysis. Since the results were consistent with the up-regulated genes identified in the Kyoto Encyclopedia of Genes and Genomes pathway of differentially expressed genes by transcriptome sequencing analysis, this confirms the accuracy and reliability of the transcriptome sequencing data.
Biology and Life Sciences, Agricultural Science and Agronomy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
