Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Expression Profiling of Salt- And Drought-Stress Responsive Genes in Wild Barley (Hordeum spontaneum L.)

Melike Bakır
ORCID logo ,
Ahu Altınkut Uncuoğlu
,
Canan Yüksel Özmen
,
Funda Yılmaz Baydu
,
Kemal Kazan
,
Umut Kibar
,
Karen Schlauch
,
John Chandler Cushman
ORCID logo ,
Ali Ergül
*
Version 1 : Received: 3 July 2023 / Approved: 4 July 2023 / Online: 4 July 2023 (09:52:45 CEST)

How to cite: Bakır, M.; Altınkut Uncuoğlu, A.; Yüksel Özmen, C.; Yılmaz Baydu, F.; Kazan, K.; Kibar, U.; Schlauch, K.; Cushman, J.C.; Ergül, A. Expression Profiling of Salt- And Drought-Stress Responsive Genes in Wild Barley (Hordeum spontaneum L.). Preprints 2023, 2023070178. https://doi.org/10.20944/preprints202307.0178.v1 Bakır, M.; Altınkut Uncuoğlu, A.; Yüksel Özmen, C.; Yılmaz Baydu, F.; Kazan, K.; Kibar, U.; Schlauch, K.; Cushman, J.C.; Ergül, A. Expression Profiling of Salt- And Drought-Stress Responsive Genes in Wild Barley (Hordeum spontaneum L.). Preprints 2023, 2023070178. https://doi.org/10.20944/preprints202307.0178.v1

Abstract

Drought and salinity are among the most important abiotic stress factors that limit the productivity in economically important plants. Although genes from wild relatives or unadapted germplasm have often been used for stress tolerance breeding, only few studies have examined wild relatives to understand the genetic and molecular basis of stress tolerance. In this study, we monitored the changes in gene expression profiles of leaf and root tissues of two wild barleys (Hordeum spontaneum L.) accessions from Israel after treatment with salt (NaCl) and drought (PEG) stress. We identified a total of 641 differentially expressed genes across 24 conditions (2 accessions, 2 stress conditions, 2 tissues and 3 time points). Our gene expression analyses revealed large numbers of differentially expressed genes in different accessions by different stress treatments. In addition, the number of genes altered in the leaves of stress-exposed plants of both genotypes was higher than those altered in the roots. Interestingly, there was a relatively little overlap between the leaf and the root stress-responsive gene expression patterns, suggesting that different stress-associated processes might be operating in these tissues during stress adaptation. Overall, our results revealed a number of candidate genes and plant processes associated with stress tolerance in wild barley. Wild barley would be a useful source of new genetic variation for drought and salt stress tolerance. Our results could provide new insights into the mechanisms of drought and salt stress tolerance in wild barley and should be useful for genetic improvement of salt-drought tolerance in cultivated barley.

Keywords

wild barley; (Hordeum spontaneum L.),; drought and salinity; transcriptome analysis

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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