Version 1
: Received: 2 October 2021 / Approved: 4 October 2021 / Online: 4 October 2021 (15:01:07 CEST)
How to cite:
Ghanbari Moheb Seraj, R.; Tohidfar, M.; Ahmadikhah, A. Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints2021, 2021100062. https://doi.org/10.20944/preprints202110.0062.v1
Ghanbari Moheb Seraj, R.; Tohidfar, M.; Ahmadikhah, A. Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints 2021, 2021100062. https://doi.org/10.20944/preprints202110.0062.v1
Ghanbari Moheb Seraj, R.; Tohidfar, M.; Ahmadikhah, A. Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints2021, 2021100062. https://doi.org/10.20944/preprints202110.0062.v1
APA Style
Ghanbari Moheb Seraj, R., Tohidfar, M., & Ahmadikhah, A. (2021). Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints. https://doi.org/10.20944/preprints202110.0062.v1
Chicago/Turabian Style
Ghanbari Moheb Seraj, R., Masoud Tohidfar and Asadollah Ahmadikhah. 2021 "Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress" Preprints. https://doi.org/10.20944/preprints202110.0062.v1
Abstract
Identification of the differentially-expressed genes is important for clarification of the complex molecular mechanisms under drought conditions. In this experiment, transcriptome profiles of sensitive and tolerant tomato genotypes under drought stress were analyzed. Three up-regulated genes were selected, included CAB3 (Chlorophyll a-b binding protein3), SAMDC (S-adenosylmethionine decarboxylase proenzyme), and ACS9 (1-aminocyclopropane-1-carboxylate synthase 9). After bioinformatics analysis, tomato genotypes were subjected to drought stress and gene expression was determined using Real-Time-PCR. Physiological parameters of genotypes were also measured by spectrophotometer-based methods. According to the results, these three genes play a key role in stress tolerance. Expression of the CAB3 gene in both sensitive and tolerant genotypes was not significantly different compared to the control, but the SAMDC gene decreased in both genotypes and the ACS9 gene decreased in sensitive genotype and increased in tolerant genotype. The physiological analysis also showed that under stress conditions, the photosynthetic system of the plant was disrupted and the chlorophyll content was reduced, but, proline content and antioxidant enzymes activity increased, in which their quantity in the tolerant genotype was significantly higher than sensitive. Under drought stress, due to damage to the lipid membrane, Malondialdehyde content also increased, in which the sensitive genotype was more affected.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
