PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial
Version 1
: Received: 26 January 2024 / Approved: 29 January 2024 / Online: 29 January 2024 (08:51:54 CET)
How to cite:
Abbaszadeh, S.H.; Yousefi, M.; Arefhosseini, S.R.; Mahmoodpoor, A.; Ebrahimi-Mameghani, M. The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial. Preprints2024, 2024011988. https://doi.org/10.20944/preprints202401.1988.v1
Abbaszadeh, S.H.; Yousefi, M.; Arefhosseini, S.R.; Mahmoodpoor, A.; Ebrahimi-Mameghani, M. The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial. Preprints 2024, 2024011988. https://doi.org/10.20944/preprints202401.1988.v1
Abbaszadeh, S.H.; Yousefi, M.; Arefhosseini, S.R.; Mahmoodpoor, A.; Ebrahimi-Mameghani, M. The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial. Preprints2024, 2024011988. https://doi.org/10.20944/preprints202401.1988.v1
APA Style
Abbaszadeh, S.H., Yousefi, M., Arefhosseini, S.R., Mahmoodpoor, A., & Ebrahimi-Mameghani, M. (2024). The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial. Preprints. https://doi.org/10.20944/preprints202401.1988.v1
Chicago/Turabian Style
Abbaszadeh, S.H., Ata Mahmoodpoor and Mehrangiz Ebrahimi-Mameghani. 2024 "The Effect of Seven-Strain Probiotic Supplementation on Th17 and Treg in Patients with Severe Traumatic Brain Injury Admitted to ICU: A Randomized Controlled Clinical Trial" Preprints. https://doi.org/10.20944/preprints202401.1988.v1
Abstract
This study investigated the impact of seven-strain probiotic supplementation on the frequency of T helper 17 (Th17) cells, regulatory T (T-reg) cells, Treg/Th17 ratio, and their gene expression in individuals with severe traumatic brain injury (STBI). Probiotics can influence the gut microbiota, modulate the immune response, and prevent or alleviate certain immune diseases. However, it is necessary to clarify whether probiotics can effectively impact Th cells in STBI patient. Despite the great attention to improving the immune system in most diseases, there is currently no accurate mechanism for the use of probiotics in diseases with damage to the head injury, which can be a basis for the use of probiotics. The primary objective of this study is to address this research gap. The present study was a randomized, double-blind, placebo-controlled trial that enrolled 48 STBI patients. They were treated with probiotics or placebo: 4 capsule/day for 14 days. The pre- and post-study evaluation included examining the frequency of Treg, Th17 cells, as well as the expression of associated immunological parameters (Foxp3 and RORγt) and Treg/Th17 ratio in both groups. No significant differences were found between the two groups in terms of subject characteristics (P>0.05). GCS scores at 14 days were significantly higher in the probiotic compared to the placebo group. The probiotic group showed a significant decrease in Th17 cell and RORγt gene expression (P = 0.022 and P< 0.001, respectively), a notable increase in T-reg and FoxP3 gene expression (P= 0.025 and P< 0.001, respectively), and a higher Treg/Th17 ratio compared to the placebo group. Our study provides promising evidence of the beneficial effects of seven-strain probiotic supplementation on Th cells in individuals with STBI.
Keywords
severe traumatic brain injury; regulatory T cell; forkhead box protein 3; T helper 17; retinoic acid receptor-related orphan receptor gamma t; gut microbiome; probiotic
Subject
Medicine and Pharmacology, Clinical Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
