Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Developmental Formation of the GABAergic and Glycinergic Networks in the Mouse Spinal Cord

Version 1 : Received: 28 December 2021 / Approved: 29 December 2021 / Online: 29 December 2021 (14:27:41 CET)

A peer-reviewed article of this Preprint also exists.

Shimizu-Okabe, C.; Kobayashi, S.; Kim, J.; Kosaka, Y.; Sunagawa, M.; Okabe, A.; Takayama, C. Developmental Formation of the GABAergic and Glycinergic Networks in the Mouse Spinal Cord. Int. J. Mol. Sci. 2022, 23, 834. Shimizu-Okabe, C.; Kobayashi, S.; Kim, J.; Kosaka, Y.; Sunagawa, M.; Okabe, A.; Takayama, C. Developmental Formation of the GABAergic and Glycinergic Networks in the Mouse Spinal Cord. Int. J. Mol. Sci. 2022, 23, 834.

Journal reference: Int. J. Mol. Sci. 2022, 23, 834
DOI: 10.3390/ijms23020834

Abstract

Gamma-aminobutyric acid (GABA) and glycine act as inhibitory neurotransmitters. Three types of inhibitory neurons and terminals, GABAergic, GABA/glycine co-releasing, and glycinergic, are orchestrated in the spinal cord neural circuits and play key roles in the regulation of pain, locomotive movement, and respiratory rhythms. Herein, we first describe GABAergic and glycinergic transmission and inhibitory networks, which consist of three types of terminals, in the mature mouse spinal cord. Second, we describe the developmental formation of GABAergic and glycinergic networks, with specific focus on the differentiation of neurons, formation of synapses, maturation of removal systems, and changes in their action. GABAergic and glycinergic neurons are derived from the same domains of the ventricular zone. Initially, GABAergic neurons are differentiated and their axons form synapses. Some of these neurons remain GABAergic in lamina I and II. Many of GABAergic neurons convert to co-releasing state. The co-releasing neurons and terminals remain in the dorsal horn, whereas many of co-releasing ones ultimately become glycinergic in the ventral horn. During the development of terminals and the transformation from radial glia to astrocytes, GABA and glycine receptor subunit compositions markedly change, removal systems mature, and GABAergic and glycinergic action shifts from excitatory to inhibitory.

Keywords

astrocyte; gamma-amino butyric acid (GABA); GABA transporter (GAT); GABAA receptor; glutamic acid decarboxylase (GAD); glycine; glycine receptor; glycine transporter (GlyT); K+-Cl- co-transporter 2 (KCC2); vesicular GABA transporter (VGAT)

Subject

MEDICINE & PHARMACOLOGY, General Medical Research

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.