Version 1
: Received: 22 May 2024 / Approved: 22 May 2024 / Online: 22 May 2024 (08:56:53 CEST)
How to cite:
Mahapatra, C.; Thakkar, R. In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity. Preprints2024, 2024051429. https://doi.org/10.20944/preprints202405.1429.v1
Mahapatra, C.; Thakkar, R. In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity. Preprints 2024, 2024051429. https://doi.org/10.20944/preprints202405.1429.v1
Mahapatra, C.; Thakkar, R. In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity. Preprints2024, 2024051429. https://doi.org/10.20944/preprints202405.1429.v1
APA Style
Mahapatra, C., & Thakkar, R. (2024). In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity. Preprints. https://doi.org/10.20944/preprints202405.1429.v1
Chicago/Turabian Style
Mahapatra, C. and Ravindra Thakkar. 2024 "In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity" Preprints. https://doi.org/10.20944/preprints202405.1429.v1
Abstract
Enhanced electrical activity in detrusor smooth muscle (DSM) cells is a key factor in detrusor overactivity which causes overactive bladder pathological disorders. Transient receptor potential melastatin-4 (TRPM4) channels, calcium-activated cation channels, play a role in regulating DSM electrical activities. These channels likely contribute to depolarizing the DSM cell membrane, leading to bladder overactivity. Our research focuses on understanding TRPM4 channel function in mouse DSM cells using computational modeling. We aimed to create a detailed computational model of the TRPM4 channel based on existing electrophysiological data. We employed a modified Hodgkin-Huxley model with an incorporated TRP-like current to simulate action potential firing in response to current and synaptic stimulus inputs. Validation against experimental data showed close agreement with our simulations. Our model is the first to analyze the TRPM4 channel's role in DSM electrical activity, potentially revealing insights into bladder overactivity. In conclusion, TRPM4 channels are pivotal in regulating human DSM function and TRPM4 channel inhibitors could be promising targets for treating overactive bladder.
Keywords
urinary incontinence; TRPM4 ion channel; action potential; computational modeling
Subject
Medicine and Pharmacology, Urology and Nephrology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
