PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2)
Version 1
: Received: 17 May 2024 / Approved: 17 May 2024 / Online: 17 May 2024 (16:01:10 CEST)
How to cite:
Patel, S.; Rai, S. Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2). Preprints2024, 2024051197. https://doi.org/10.20944/preprints202405.1197.v1
Patel, S.; Rai, S. Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2). Preprints 2024, 2024051197. https://doi.org/10.20944/preprints202405.1197.v1
Patel, S.; Rai, S. Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2). Preprints2024, 2024051197. https://doi.org/10.20944/preprints202405.1197.v1
APA Style
Patel, S., & Rai, S. (2024). Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2). Preprints. https://doi.org/10.20944/preprints202405.1197.v1
Chicago/Turabian Style
Patel, S. and Seema Rai. 2024 "Melatonin Takes the Edge off Dexamethasone-Induced Testicular Damage via Modulating Antioxidative Enzymes and Interactive Dialogues between Androgen (AR) and Melatonin Receptors (Mt1/Mt2)" Preprints. https://doi.org/10.20944/preprints202405.1197.v1
Abstract
The present study aims to elucidate the effect of melatonin (MEL) on dexamethasone (DEX)-induced reproductive impairments in rats. Rats were procured and acclimatized with adequate food and water ad libitum. Twenty-four rats were divided into four groups (six in each) of Control, DEX (70µg/100g bw), DEX plus MEL, and MEL (200µg/100g bw) for 21 days. After completion, rats were sacrificed, and testes were dissected, weighed, and processed for biochemical, and receptor expression. Serum was collected for hormonal assay. DEX-treated rats showed a significant increase in lipid peroxidation (TBARS), a decrease in weight of the body and testes, and the level of antioxidative enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione oxidase (GSH), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH), glutathione S-transferase (GST)), total cellular protein content, serum testosterone, corticosterone, LH, FSH, melatonin levels, AR, MT1, and MT2 receptor expression. Marked cellular alterations were noted in the testis histology of the DEX group. Melatonin treated in DEX rats showed recovery and restoration in gravimetric, serum hormonal, biochemical parameters, receptor expression, and cellularity of testes. Therefore, findings suggest that melatonin improved defence against DEX-induced testicular damages suggesting its therapeutic potential for the management of male reproductive health.
Keywords
melatonin receptor; androgen receptor; oxidative stress; male infertility; antioxidant enzyme
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
