Version 1
: Received: 14 May 2024 / Approved: 15 May 2024 / Online: 15 May 2024 (07:48:20 CEST)
How to cite:
Shi, M.; He, Y.; Zheng, J.; Xu, Y.; Tan, Y.; Jia, L.; Chen, L.; Ye, J.; Qi, C. Effects of Soybean Isoflavone on the Growth Performance and Lipid Metabolism of Juvenile Chinese Mitten Crab Eriocheir sinensis. Preprints2024, 2024051013. https://doi.org/10.20944/preprints202405.1013.v1
Shi, M.; He, Y.; Zheng, J.; Xu, Y.; Tan, Y.; Jia, L.; Chen, L.; Ye, J.; Qi, C. Effects of Soybean Isoflavone on the Growth Performance and Lipid Metabolism of Juvenile Chinese Mitten Crab Eriocheir sinensis. Preprints 2024, 2024051013. https://doi.org/10.20944/preprints202405.1013.v1
Shi, M.; He, Y.; Zheng, J.; Xu, Y.; Tan, Y.; Jia, L.; Chen, L.; Ye, J.; Qi, C. Effects of Soybean Isoflavone on the Growth Performance and Lipid Metabolism of Juvenile Chinese Mitten Crab Eriocheir sinensis. Preprints2024, 2024051013. https://doi.org/10.20944/preprints202405.1013.v1
APA Style
Shi, M., He, Y., Zheng, J., Xu, Y., Tan, Y., Jia, L., Chen, L., Ye, J., & Qi, C. (2024). Effects of Soybean Isoflavone on the Growth Performance and Lipid Metabolism of Juvenile Chinese Mitten Crab <em>Eriocheir sinensis</em>. Preprints. https://doi.org/10.20944/preprints202405.1013.v1
Chicago/Turabian Style
Shi, M., Jinyun Ye and Changle Qi. 2024 "Effects of Soybean Isoflavone on the Growth Performance and Lipid Metabolism of Juvenile Chinese Mitten Crab <em>Eriocheir sinensis</em>" Preprints. https://doi.org/10.20944/preprints202405.1013.v1
Abstract
In order to study the effects of soybean isoflavones on the growth performance and lipid metabolism of juvenile Chinese mitten crab, six experimental diets were formulated by gradient supplementation 0%, 0.004% and 0.008% soybean isoflavones at different dietary lipid levels (10% and 15%), which were named to NF-0 group (10% fat and 0% SIF), NF-0.004 group (10% fat and 0.004% SIF), NF-0.008 group (10% fat and 0.008% SIF), HF-0 group (15% fat and 0% SIF), HF-0.004 group (15% fat and 0.004% SIF) and HF-0.008 group (15% fat and 0.008% SIF). All crabs with an initial weight of 0.4 ± 0.03 g were fed for 8 weeks. The results showed that diets supplementation with 0.004% or 0.008% SIF significantly increased the weight gain and specific growth rate of crabs. Diets supplementation with 0.004% or 0.008% SIF significantly reduced the content of non-esterified free fatty acids and triglycerides in the hepatopancreas of crabs at 10% dietary lipid level. Dietary SIF significantly decreased the relative mRNA expressions of elongase of very long-chain fatty acids 6 (elovl6), triglyceride lipase (tgl), sterol regulatory element binding protein 1 (srebp-1), carnitine palmitoyltransferase-1a (cpt-1a), fatty acid transporter protein 4 (fatp4), carnitine palmitoyltransferase-2 (cpt-2), Δ9 fatty acyl desaturase (Δ9 fad), carnitine palmitoyltransferase-1b (cpt-1b), fatty acid binding protein 10 (fabp10) and microsomal triglyceride transfer protein (mttp) in the hepatopancreas of crabs. At 15% dietary lipid level, 0.008% SIF significantly increased the relative mRNA expressions of fatty acid binding protein 3 (fabp3), carnitine acetyltransferase (caat), fatp4, fabp10, tgl, cpt-1a, cpt-1b and cpt-2 and significantly down regulated the relative mRNA expressions of Δ9 fad and srebp-1. In conclusion, SIF can improve the growth and utilization of high-fat diet by inhibiting genes related to lipid synthesis and promoting lipid decomposition in juvenile Chinese mitten crab.
Keywords
Soybean isoflavones; Chinese mitten crab; Growth performance; Lipid metabolism
Subject
Biology and Life Sciences, Aquatic Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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