Version 1
: Received: 14 May 2024 / Approved: 14 May 2024 / Online: 14 May 2024 (13:06:45 CEST)
How to cite:
Kim, Y. K.; Cho, M. J.; Kang, D.-J. Anti-inflammatory Response of a New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-Like HaCaT Keratinocytes. Preprints2024, 2024050949. https://doi.org/10.20944/preprints202405.0949.v1
Kim, Y. K.; Cho, M. J.; Kang, D.-J. Anti-inflammatory Response of a New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-Like HaCaT Keratinocytes. Preprints 2024, 2024050949. https://doi.org/10.20944/preprints202405.0949.v1
Kim, Y. K.; Cho, M. J.; Kang, D.-J. Anti-inflammatory Response of a New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-Like HaCaT Keratinocytes. Preprints2024, 2024050949. https://doi.org/10.20944/preprints202405.0949.v1
APA Style
Kim, Y. K., Cho, M. J., & Kang, D. J. (2024). Anti-inflammatory Response of a New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-Like HaCaT Keratinocytes. Preprints. https://doi.org/10.20944/preprints202405.0949.v1
Chicago/Turabian Style
Kim, Y. K., Min Ji Cho and Dae-Jung Kang. 2024 "Anti-inflammatory Response of a New Postbiotics in TNF-α/IFN-γ-Induced Atopic Dermatitis-Like HaCaT Keratinocytes" Preprints. https://doi.org/10.20944/preprints202405.0949.v1
Abstract
This study examines the synergistic interaction between the immunomodulatory functions of lactic acid bacteria postbiotics and the anti-inflammatory properties of Smilax China L. extract through a combined fermentation process. Using atopic dermatitis (AD) as a model, characterized by an immune imbalance that leads to skin inflammation, we developed a fermented product, MB-2006, and compared its effects to those of heat-killed probiotics, Lactobacillus acidophilus (LAC) and Lac-tobacillus rhamnosus (LRH). Our experiments focused on elucidating the mechanism of action of MB-2006 in AD-like HaCaT keratinocyte cells, particularly its impact on the NF-kB pathway, a pivotal regulator of inflammation. MB-2006 proved more effective in reducing inflammation markers such as IL-4 and thymic stromal lymphopoietin (TSLP), and in inhibiting NF-kB activation, compared to LAC and LRH. Significantly, MB-2006 also reduced the expression of thymus- and activation-regulated chemokine (TARC), highlighting a synergistic effect that enhances its thera-peutic potential. These results suggest that the combined fermentation of Smilax china L. extract with lactic acid bacteria enhanced both the anti-inflammatory and immunomodulatory effects, pre-senting a promising integrative approach to treating conditions like AD. Further studies are needed to validate these results in clinical settings and fully explore the potential of this synergistic fer-mentation process.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
