Version 1
: Received: 29 April 2024 / Approved: 29 April 2024 / Online: 30 April 2024 (10:12:12 CEST)
How to cite:
Cheng, M.; Janzekovic, J.; Finze, R.; Mohseni, M.; Saifzadeh, S.; Ung, O.; Wagels, M.; Hutmacher, D. W. Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model. Preprints2024, 2024041948. https://doi.org/10.20944/preprints202404.1948.v1
Cheng, M.; Janzekovic, J.; Finze, R.; Mohseni, M.; Saifzadeh, S.; Ung, O.; Wagels, M.; Hutmacher, D. W. Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model. Preprints 2024, 2024041948. https://doi.org/10.20944/preprints202404.1948.v1
Cheng, M.; Janzekovic, J.; Finze, R.; Mohseni, M.; Saifzadeh, S.; Ung, O.; Wagels, M.; Hutmacher, D. W. Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model. Preprints2024, 2024041948. https://doi.org/10.20944/preprints202404.1948.v1
APA Style
Cheng, M., Janzekovic, J., Finze, R., Mohseni, M., Saifzadeh, S., Ung, O., Wagels, M., & Hutmacher, D. W. (2024). Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model. Preprints. https://doi.org/10.20944/preprints202404.1948.v1
Chicago/Turabian Style
Cheng, M., Michael Wagels and Dietmar W Hutmacher. 2024 "Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model" Preprints. https://doi.org/10.20944/preprints202404.1948.v1
Abstract
Scaffold guided breast tissue regeneration (SGBTR) can both transform reconstructive and cosmetic breast surgery. Implant-based surgery is the most common method. However, there are inherent limitations as there is replacement of tissue rather than regeneration. Regenerating autologous soft tissue has the potential to provide a more like-for-like reconstruction with minimal morbidity. Our SGBTR approach regenerates soft tissue by implanting additively manufactured bioresorbable scaffolds filled with autologous fat graft. A pre-clinical large animal study was conducted implanting 100 ml breast scaffolds (n=55) made from medical-grade polycaprolactone in 11 minipigs for 12 months. Various treatment groups were investigated where immediate or delayed autologous fat graft, as well as platelet rich plasma were added to scaffolds. Computed Tomography and Magnetic Resonance Imaging was performed on explanted scaffolds to determine volume and distribution of regenerated tissue. Histological analysis was performed to confirm tissue type. At 12 months we were able to regenerate and sustain a mean soft tissue volume of 60.9 4.5 ml (95% CI) across all treatment groups. There was no evidence of capsule formation. There were no immediate or long-term post-operative complications. In conclusion, we were able to regenerate clinically relevant soft tissue volumes utilizing SGBTR in a pre-clinical large animal model.
Keywords
Tissue engineering; regenerative medicine; breast reconstruction; scaffold; polycaprolactone; soft tissue; adipose tissue; pre-clinical; large animal; pig
Subject
Medicine and Pharmacology, Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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