Version 1
: Received: 19 April 2024 / Approved: 22 April 2024 / Online: 23 April 2024 (08:10:04 CEST)
Version 2
: Received: 23 April 2024 / Approved: 24 April 2024 / Online: 24 April 2024 (14:11:35 CEST)
How to cite:
Hanafi, D.; Onyenwoke, R.U.; Kimbro, K.S. The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines. Preprints2024, 2024041453. https://doi.org/10.20944/preprints202404.1453.v1
Hanafi, D.; Onyenwoke, R.U.; Kimbro, K.S. The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines. Preprints 2024, 2024041453. https://doi.org/10.20944/preprints202404.1453.v1
Hanafi, D.; Onyenwoke, R.U.; Kimbro, K.S. The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines. Preprints2024, 2024041453. https://doi.org/10.20944/preprints202404.1453.v1
APA Style
Hanafi, D., Onyenwoke, R.U., & Kimbro, K.S. (2024). The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines. Preprints. https://doi.org/10.20944/preprints202404.1453.v1
Chicago/Turabian Style
Hanafi, D., Rob U Onyenwoke and K Sean Kimbro. 2024 "The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines" Preprints. https://doi.org/10.20944/preprints202404.1453.v1
Abstract
The G-protein-coupled estrogen receptor (GPER; G protein-coupled estrogen receptor 30, also known as GPR30) is a novel estrogen receptor and has emerged as a promising target for ovarian cancer. GPER, a seven-transmembrane receptor, suppresses cellular viability and migration in studied ovarian cancer cells. However, its impact on the fallopian tube, which is the potential origin of high-grade serous (HGSC) ovarian cancer, has not been addressed. This study was conducted to evaluate the relationship of GPER, ovarian cancer subtypes, i.e., high-grade serous cell lines (OV90 and OVCAR420), as well as potential HGSC ovarian cancer origin (i.e., the fallopian tube cell line FT190). The selective ligand assessed here is the agonist G-1, which was utilized in an in vitro study to characterize its effects on cellular viability and migration. As a result, this study has addressed the effect of a specific GPER antagonist in combination with an agonist on cell viability, providing a better understanding of the effects of these compounds on our diverse group of studied cell lines. Strikingly, attenuated cell proliferation and migration behaviors were observed in the presence of G-1. Thus, our in vitro study reveals the impact of the origin of HGSC ovarian cancers and highlights the GPER agonist G-1 as a potential therapy for ovarian cancer.
Keywords
GPER receptor; Therapeutic; Fallopian Tubes; Ovarian Cancer
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
