Version 1
: Received: 18 April 2024 / Approved: 18 April 2024 / Online: 18 April 2024 (11:36:10 CEST)
How to cite:
Rychtarova, J.; Fulka, H.; Loi, P.; Fulka, Jr., J. Assessing the Sperm Head DNA Damage in Frozen/Thawed Horse Semen via Interspecific ICSI. Preprints2024, 2024041256. https://doi.org/10.20944/preprints202404.1256.v1
Rychtarova, J.; Fulka, H.; Loi, P.; Fulka, Jr., J. Assessing the Sperm Head DNA Damage in Frozen/Thawed Horse Semen via Interspecific ICSI. Preprints 2024, 2024041256. https://doi.org/10.20944/preprints202404.1256.v1
Rychtarova, J.; Fulka, H.; Loi, P.; Fulka, Jr., J. Assessing the Sperm Head DNA Damage in Frozen/Thawed Horse Semen via Interspecific ICSI. Preprints2024, 2024041256. https://doi.org/10.20944/preprints202404.1256.v1
APA Style
Rychtarova, J., Fulka, H., Loi, P., & Fulka, Jr., J. (2024). Assessing the Sperm Head DNA Damage in Frozen/Thawed Horse Semen via Interspecific ICSI. Preprints. https://doi.org/10.20944/preprints202404.1256.v1
Chicago/Turabian Style
Rychtarova, J., Pasqualino Loi and Josef Fulka, Jr.. 2024 "Assessing the Sperm Head DNA Damage in Frozen/Thawed Horse Semen via Interspecific ICSI" Preprints. https://doi.org/10.20944/preprints202404.1256.v1
Abstract
The sperm heads of frozen/thawed horse spermatozoa were injected into ovulated mouse oocytes where they decondensed and formed paternal pronuclei in which the DNA damage has been evaluated. The motile spermatozoa exhibit none or minor level of DNA damage and the xenogenic zygotes cleave to the two-cell embryonic stage with regular nuclei and no micronuclei (MNs). On the other hand, the immotile spermatozoa exhibit typically high levels of DNA damage and blastomeres in two-cell stage embryos contain multiple MNs. Moreover, extremely high sperm DNA damage prevents even the cleavage to the two-cell stage.Keywords: keyword 1; keyword 2; keyword 3 (List three to ten pertinent keywords specific to the article yet reasonably common within the subject discipline.)
Keywords
horse; spermatozoa; freezing; ICSI; DNA
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
