Version 1
: Received: 17 April 2024 / Approved: 17 April 2024 / Online: 17 April 2024 (11:51:39 CEST)
How to cite:
YANG, G.; Zhang, J.; LIU, Y.; SUN, J.; GE, L.; LU, L.; LONG, K.; LI, X.; XU, D.; MA, J. Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice. Preprints2024, 2024041146. https://doi.org/10.20944/preprints202404.1146.v1
YANG, G.; Zhang, J.; LIU, Y.; SUN, J.; GE, L.; LU, L.; LONG, K.; LI, X.; XU, D.; MA, J. Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice. Preprints 2024, 2024041146. https://doi.org/10.20944/preprints202404.1146.v1
YANG, G.; Zhang, J.; LIU, Y.; SUN, J.; GE, L.; LU, L.; LONG, K.; LI, X.; XU, D.; MA, J. Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice. Preprints2024, 2024041146. https://doi.org/10.20944/preprints202404.1146.v1
APA Style
YANG, G., Zhang, J., LIU, Y., SUN, J., GE, L., LU, L., LONG, K., LI, X., XU, D., & MA, J. (2024). Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice. Preprints. https://doi.org/10.20944/preprints202404.1146.v1
Chicago/Turabian Style
YANG, G., Dengfeng XU and Jideng MA. 2024 "Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice" Preprints. https://doi.org/10.20944/preprints202404.1146.v1
Abstract
The normal growth and development of skeletal muscle is essential for the health of the body. The regulation of skeletal muscle by intestinal microorganisms and their metabolites has been continuously revealed. Acetate is the predominant short-chain fatty acid synthesized by gut microbiota through the fermentation of dietary fiber, however, the underlying molecular mechanisms governing the interaction between acetate and skeletal muscle during rapid growth stage remain to be further elucidated; Here, specific pathogen-free (SPF) mice, germ-free (GF) mice, and germ-free mice supplemented with sodium acetate (GS) were used to evaluate the effects of acetate on skeletal muscle growth and development of young mice with gut microbiota deficiency; We found the concentration of serum acetate, body mass gain, succinate dehydrogenase activity and expression of myogenesis maker gene of skeletal muscle in the GS group was higher than GF group following sodium acetate supplement. Furthermore, the transcriptome analysis revealed that acetate activated biological processes regulating skeletal muscle growth and development in GF group that are inhibited due to gut microbiota deficiency. In vitro experiment, we proved that acetate up-regulated Gm16062 to promote skeletal muscle cell differentiation; Overall, our findings proved that acetate promote skeletal muscle growth and development in young mice via increase Gm16062 expression.
Keywords
gut microbiota, acetate, skeletal muscle, Gm16062,
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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