Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Effects of Novel miRNAs Found in Exosome Vesicles Derived from Cord Blood Stem Cells (CBSCs-EVs) on CHL1 Melanoma Cells Compared to Healthy Control

Mojgan Najafzadeh
* ,
Shohreh Jafarinejad
,
Sajad Falsafi Zadeh
,
Adi Baumgartner
,
Mohammad Isreb
ORCID logo ,
Pouria Akhbari
ORCID logo ,
Nader Ghaderi
,
Farshid Sefat
ORCID logo ,
Saeed Heidari Keshel
,
Rojan Ghaderi
,
Diana Anderson
,
Andrew Wright
Version 1 : Received: 17 April 2024 / Approved: 17 April 2024 / Online: 17 April 2024 (08:55:39 CEST)

How to cite: Najafzadeh, M.; Jafarinejad, S.; Falsafi Zadeh, S.; Baumgartner, A.; Isreb, M.; Akhbari, P.; Ghaderi, N.; Sefat, F.; Keshel, S.H.; Ghaderi, R.; Anderson, D.; Wright, A. The Effects of Novel miRNAs Found in Exosome Vesicles Derived from Cord Blood Stem Cells (CBSCs-EVs) on CHL1 Melanoma Cells Compared to Healthy Control. Preprints 2024, 2024041128. https://doi.org/10.20944/preprints202404.1128.v1 Najafzadeh, M.; Jafarinejad, S.; Falsafi Zadeh, S.; Baumgartner, A.; Isreb, M.; Akhbari, P.; Ghaderi, N.; Sefat, F.; Keshel, S.H.; Ghaderi, R.; Anderson, D.; Wright, A. The Effects of Novel miRNAs Found in Exosome Vesicles Derived from Cord Blood Stem Cells (CBSCs-EVs) on CHL1 Melanoma Cells Compared to Healthy Control. Preprints 2024, 2024041128. https://doi.org/10.20944/preprints202404.1128.v1

Abstract

This study aimed to investigate the potential of Cord Blood Stem Cell-derived exosome vesicles (CBSC-EVs) in regulating cancer growth and preventing cellular damage. The study aimed to examine the effects of CBSC-EVs on CHL1 cancer cells and healthy fibroblasts. The study results reveal that CBSC-EVs have an anti-cancer/antioxidant effect on CHL1 cancer cells without inducing any toxic effects on healthy lymphocytes. Further analysis of CBSC-EVs revealed the presence of seven miRNAs (noncoding RNA molecules), including three from the Let 7 family, two from the Let 5 family, and two novel miRNAs. The function of the novel miRNAs was the main focus of the study. To assess the efficacy of CBSC-EVs, CHL1 cells (a type of malignant melanoma cell line) and human fibroblasts were grouped into different treatments. One group of those cell lines were treated with CBSC-EVs, and in the second group, the cells were transfected with either miRNA inhibitor Novel 1 (miRNA IN1) plus CBSC-EVs or miRNA inhibitor Novel 2 (miRNA IN2) and CBSC-EVs to knock down those novel miRNAs in the CBSC-EVs. Therefore, by comparing these groups, the function of novel miRNAs is more understandable. In this study, the Comet assay was employed to evaluate cancer cells' DNA damage and CCK8 to measure cell viability. The findings revealed that CBSC-EVs caused an increase in DNA damage in CHL1 cells compared to cells without treatment (p

Keywords

miRNA; Apoptosis; cytotoxic; CBSC-derived exosomes; CHL1 malignant melanoma; fibroblast; lymphocytes

Subject

Biology and Life Sciences, Other

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.