Version 1
: Received: 7 April 2024 / Approved: 16 April 2024 / Online: 17 April 2024 (11:24:11 CEST)
How to cite:
Zhang, Q.; Bu, B.; Zhu, Y.; Li, J.; Luo, M.; Deng, L.; Ouyang, M. α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions. Preprints2024, 2024041106. https://doi.org/10.20944/preprints202404.1106.v1
Zhang, Q.; Bu, B.; Zhu, Y.; Li, J.; Luo, M.; Deng, L.; Ouyang, M. α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions. Preprints 2024, 2024041106. https://doi.org/10.20944/preprints202404.1106.v1
Zhang, Q.; Bu, B.; Zhu, Y.; Li, J.; Luo, M.; Deng, L.; Ouyang, M. α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions. Preprints2024, 2024041106. https://doi.org/10.20944/preprints202404.1106.v1
APA Style
Zhang, Q., Bu, B., Zhu, Y., Li, J., Luo, M., Deng, L., & Ouyang, M. (2024). α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions. Preprints. https://doi.org/10.20944/preprints202404.1106.v1
Chicago/Turabian Style
Zhang, Q., Linhong Deng and Mingxing Ouyang. 2024 "α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions" Preprints. https://doi.org/10.20944/preprints202404.1106.v1
Abstract
Cell-to-cell distant mechanical communication has been demonstrated by using in-vitro and in-vivo models. However, the molecular mechanisms underlying long-range cell mechanoresponsive interactions remain to be more elucidated. This study further examined the roles of α-Catenin and Piezo in traction force-induced rapid branch assembly of airway smooth muscle (ASM) cells on Matrigel hydrogel containing type I collagen. Our findings demonstrate that siRNA-mediated downregulation of α-Catenin or chemical inhibition of Piezo activity significantly reduced both cell directional movement and branching assembly. In regarding the role of N-cadherin in regulating branch assembly but not directional migration, our results further confirmed that siRNA downregulation of α-Catenin caused a remarked reduction of focal adhesion formation, as assessed by focal Paxillin and Integrin α5 localization. These observations implied that mechanosensitive α-Catenin was involved in both cell-cell and cell-matrix adhesions. Additionally, Piezo showed partial localization with Paxillin in focal adhesions, which was inhibited by α-Catenin downregulation with siRNA. This provides a plausible clue for Piezo mechanosensing traction force in the hydrogel. Collectively, our findings highlight the significance of α-Catenin in regulating cell-matrix interactions along with possible interpretation for Piezo mechanosensation at focal adhesions during cell-cell distant mechanical communication.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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