Version 1
: Received: 12 April 2024 / Approved: 15 April 2024 / Online: 16 April 2024 (07:23:40 CEST)
Version 2
: Received: 16 April 2024 / Approved: 17 April 2024 / Online: 17 April 2024 (11:59:30 CEST)
How to cite:
Collins, C.; Brown, J.; Berkley, N. MuhdoAge: A Novel Saliva Based Epigenetic Clock that Has a Strong Association with Ageing in a Healthy, Disease‐Free Cohort. Preprints2024, 2024040985. https://doi.org/10.20944/preprints202404.0985.v1
Collins, C.; Brown, J.; Berkley, N. MuhdoAge: A Novel Saliva Based Epigenetic Clock that Has a Strong Association with Ageing in a Healthy, Disease‐Free Cohort. Preprints 2024, 2024040985. https://doi.org/10.20944/preprints202404.0985.v1
Collins, C.; Brown, J.; Berkley, N. MuhdoAge: A Novel Saliva Based Epigenetic Clock that Has a Strong Association with Ageing in a Healthy, Disease‐Free Cohort. Preprints2024, 2024040985. https://doi.org/10.20944/preprints202404.0985.v1
APA Style
Collins, C., Brown, J., & Berkley, N. (2024). MuhdoAge: A Novel Saliva Based Epigenetic Clock that Has a Strong Association with Ageing in a Healthy, Disease‐Free Cohort. Preprints. https://doi.org/10.20944/preprints202404.0985.v1
Chicago/Turabian Style
Collins, C., James Brown and Nathan Berkley. 2024 "MuhdoAge: A Novel Saliva Based Epigenetic Clock that Has a Strong Association with Ageing in a Healthy, Disease‐Free Cohort" Preprints. https://doi.org/10.20944/preprints202404.0985.v1
Abstract
This study introduces the MuhdoAge clock, a novel saliva-based epigenetic clock, designed to predict biological age with strong correlations in a healthy, disease-free cohort. Leveraging a comprehensive dataset from Muhdo Health, this research analyses saliva samples utilising the Illumina MethylationEPIC array. Through robust statistical methods, 237 significant CpG sites were identified that correlate with age-related methylation changes, exhibiting a coefficient of determination (R²) of 0.652 after outlier removal, with tiered algorithmic design improving this further to 0.726 in a new dataset that combined pathologic and healthy individuals (n=2682). Comparatively, the MuhdoAge clock was evaluated against other known epigenetic clocks, demonstrating high precision in predicting biological age within healthy individuals (R² = 0.878, n=1844), emphasising its potential utility in non-invasive, large-scale applications. The clock shows promise for integration into public health strategies for monitoring and potentially mitigating age-related decline, facilitated by its ease of use and accessibility through saliva sampling. Furthermore, this study highlights the impact of lifestyle and environmental factors on biological ageing, highlighting the importance of personalised health interventions. Future research is to focus on longitudinal studies to validate these findings and explore the mechanisms underlying epigenetic age acceleration.
Keywords
Ageing; Epigenetics; Biological clock; DNA methylation; Longevity
Subject
Biology and Life Sciences, Aging
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
