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Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future
Version 1
: Received: 29 March 2024 / Approved: 29 March 2024 / Online: 29 March 2024 (13:53:44 CET)
How to cite:
Sarker, S.; Neeloy, R.M.; Habib, M.B.; Urmi, U.L.; Asad, M.A.; Mosaddek, A.S.M.; Khan, M.R.K.; Nahar, S.; Godman, B.; Islam, S. Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future. Preprints2024, 2024031830. https://doi.org/10.20944/preprints202403.1830.v1
Sarker, S.; Neeloy, R.M.; Habib, M.B.; Urmi, U.L.; Asad, M.A.; Mosaddek, A.S.M.; Khan, M.R.K.; Nahar, S.; Godman, B.; Islam, S. Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future. Preprints 2024, 2024031830. https://doi.org/10.20944/preprints202403.1830.v1
Sarker, S.; Neeloy, R.M.; Habib, M.B.; Urmi, U.L.; Asad, M.A.; Mosaddek, A.S.M.; Khan, M.R.K.; Nahar, S.; Godman, B.; Islam, S. Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future. Preprints2024, 2024031830. https://doi.org/10.20944/preprints202403.1830.v1
APA Style
Sarker, S., Neeloy, R.M., Habib, M.B., Urmi, U.L., Asad, M.A., Mosaddek, A.S.M., Khan, M.R.K., Nahar, S., Godman, B., & Islam, S. (2024). Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future. Preprints. https://doi.org/10.20944/preprints202403.1830.v1
Chicago/Turabian Style
Sarker, S., Brian Godman and Salequl Islam. 2024 "Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future" Preprints. https://doi.org/10.20944/preprints202403.1830.v1
Abstract
Abstract: Colistin is a last-resort antimicrobial for treating multidrug-resistant Gram-negative bacteria. Phe-notypic colistin resistance is highly associated with plasmid-mediated mobile colistin resistance (mcr) genes. mcr-bearing Enterobacteriaceae have been detected in many countries, with the emergence of colistin-resistant pathogens a global concern. This study assessed the distribution of mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 genes with the phenotypic colistin resistance in isolates from diarrheal infants and children in Bangladesh. Bacteria were identified using the API-20E biochemical panel and 16s rDNA gene sequencing. Polymerase chain reac-tions detected mcr gene variants in the isolates. Their susceptibilities to colistin were determined by agar dilu-tion and E-test by minimal inhibitory concentration (MIC) measurements. Over 30.0% (69/225) of isolates showed colistin resistance by agar dilution assessment (MIC> 2.0 μg/mL). Overall, 15.5% of isolates carried mcr genes (7, mcr-1; 17, mcr-2; 13, mcr-3; and co-occurrence occurred in 2 isolates). Clinical breakout MIC val-ues (≥ 4 μg/mL) were associated with 91.3% of mcr-positive isolates. The mcr-positive pathogens include twenty Escherichia spp., five Shigella flexneri, five Citrobacter spp., two Klebsiella pneumoniae, and three Pseudo-monas parafulva. mcr-genes appeared to be significantly associated with phenotypic colistin resistance phe-nomena (p=0.000), with 100% colistin-resistant isolates showing MDR phenomena. Age and sex of patients showed no significant association with detected mcr variants. Overall, mcr-associated colistin-resistant bacte-ria have emerged in Bangladesh, which warrants further research to determine their spread and instigate ac-tivities to reduce resistance.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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