PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments
Version 1
: Received: 26 February 2024 / Approved: 29 February 2024 / Online: 1 March 2024 (00:21:18 CET)
How to cite:
Yang, Q.; Zhang, M.; Sun, Y.; Zhao, B.; Cheng, Z.; Chen, L.; Zhong, Z.; Ye, Y.; Xia, Y. High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments. Preprints2024, 2024030001. https://doi.org/10.20944/preprints202403.0001.v1
Yang, Q.; Zhang, M.; Sun, Y.; Zhao, B.; Cheng, Z.; Chen, L.; Zhong, Z.; Ye, Y.; Xia, Y. High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments. Preprints 2024, 2024030001. https://doi.org/10.20944/preprints202403.0001.v1
Yang, Q.; Zhang, M.; Sun, Y.; Zhao, B.; Cheng, Z.; Chen, L.; Zhong, Z.; Ye, Y.; Xia, Y. High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments. Preprints2024, 2024030001. https://doi.org/10.20944/preprints202403.0001.v1
APA Style
Yang, Q., Zhang, M., Sun, Y., Zhao, B., Cheng, Z., Chen, L., Zhong, Z., Ye, Y., & Xia, Y. (2024). High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments. Preprints. https://doi.org/10.20944/preprints202403.0001.v1
Chicago/Turabian Style
Yang, Q., Yuhui Ye and Yu Xia. 2024 "High-throughput Analysis of Department-Specific Dynamic Patterns of Bacterial Communities and Antibiotic Resistance in the Hospital Indoor Environments" Preprints. https://doi.org/10.20944/preprints202403.0001.v1
Abstract
The hospital indoor environment plays a significant role in shaping the microbial exposures that humans encounter. Resistance to antibiotics is one of the mechanisms employed by bacteria to develop resilience against stress in indoor environments. The extensive usage of antibiotics has been a key factor in driving changes in the ecological function of antibiotic resistance genes (ARGs) and their acquisition by pathogenic bacteria. By integrating the 16S rRNA Illumina sequencing and high throughput-qPCR approaches with water and air dust samples across seven departments at Peking University Shenzhen Hospital, China, this study yielded intriguing findings regarding the department-specific variation, transmission, and correlation of bacteria, ARGs and MGEs within the hospital indoor environment. One notable observation was that the seasonal variation played a vital role in shaping the bacterial composition in the overall hospital indoor environment across seven studied departments. Nevertheless, a similar significant seasonal impact was only observed in the blood test room and emergency departments, rather than in the other five departments. Another intriguing finding was the correlation between ARGs and MGEs abundance. This correlation was evident in the overall hospital indoor environment, which was conversely not found in the emergency, pneumology and gynecology departments. Additionally, while there was no significant relationship that occurred between bacterial communities and ARGs/MGEs within the hospital, the gynecology department demonstrated a close link between bacterial communities and the presence of ARGs/MGEs. Notably, the gynecology department also stood out as an important source of bacteria and ARGs/MGEs for other departments. This study underscores the need for careful surveillance and monitoring of antibiotic resistance, particularly in Legionella spp. in man-made water systems. The findings also show the importance of monitoring and understanding the genetic elements such as Tp614 involved in gene transfer and recombination, as well as their impact on antimicrobial treatment efficacy.
Keywords
antibiotic resistance; antibiotic resistance genes; hospital indoor environment; microbial communities; high-throughput qPCR; department-specific; tap water; p-trap water; air dust; mobile gene elements
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
