PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species
Version 1
: Received: 28 February 2024 / Approved: 29 February 2024 / Online: 29 February 2024 (09:49:42 CET)
How to cite:
Muncan, J.; Tsenkova, R.; Kabayama, S.; Watanabe, Y.; Iwai, M.; Anantywittayanon, S. Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species. Preprints2024, 2024021692. https://doi.org/10.20944/preprints202402.1692.v1
Muncan, J.; Tsenkova, R.; Kabayama, S.; Watanabe, Y.; Iwai, M.; Anantywittayanon, S. Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species. Preprints 2024, 2024021692. https://doi.org/10.20944/preprints202402.1692.v1
Muncan, J.; Tsenkova, R.; Kabayama, S.; Watanabe, Y.; Iwai, M.; Anantywittayanon, S. Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species. Preprints2024, 2024021692. https://doi.org/10.20944/preprints202402.1692.v1
APA Style
Muncan, J., Tsenkova, R., Kabayama, S., Watanabe, Y., Iwai, M., & Anantywittayanon, S. (2024). Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species. Preprints. https://doi.org/10.20944/preprints202402.1692.v1
Chicago/Turabian Style
Muncan, J., Masamichi Iwai and Sukritta Anantywittayanon. 2024 "Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species" Preprints. https://doi.org/10.20944/preprints202402.1692.v1
Abstract
Reactive oxygen species (ROS) play a pivotal role in cellular function, and their overproduction contributes to oxidative stress, fatigue, aging, and diseases. To counteract these effects, incorpo-rating antioxidant-rich nutrients is recommended. Functional waters, including electrolyzed hydrogen water (EHW), are gaining attention for potential antioxidant effects through daily in-take. This study employs aquaphotomics and near-infrared spectroscopy (NIRS) to elucidate the molecular mechanisms behind the antioxidant activity of EHW. Previous investigation reported a 100% loss in antioxidant functionality through autoclaving, and a 40% decrease with hydrogen gas removal, prompting the current investigation into the mechanisms of antioxidant effects and especially the role of water molecular structure in it. Aquaphotomics analysis of near infrared (NIR) spectral data revealed that the primary characteristic of EHW associated with anti-radical function is presence of water solvation shells. Autoclaving and degassing led to loss of these water molecular species and a significant increase in free water molecules. The study suggests that water shells (solvation shells) in EHW play a crucial role in neutralizing ROS, although the specific ions or nanoparticles responsible for creating these shells remain unidentified. This concept aligns well with existing proposals of the crucial role of water solvation shells in ROS neutralization.
Keywords
electrolyzed hydrogen water; reactive oxygen species; oxidative stress; drinking water; near infrared spectroscopy; aquaphotomics; aquagram; solvation shells; free water molecules
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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