Version 1
: Received: 16 February 2024 / Approved: 18 February 2024 / Online: 19 February 2024 (11:49:06 CET)
How to cite:
Karaduman, T.; Soylukan, C. Bioactive Compounds from Campsis radicans L.: Antioxidant and Antiproliferative Effects on Colon Cancer Cells. Preprints2024, 2024020930. https://doi.org/10.20944/preprints202402.0930.v1
Karaduman, T.; Soylukan, C. Bioactive Compounds from Campsis radicans L.: Antioxidant and Antiproliferative Effects on Colon Cancer Cells. Preprints 2024, 2024020930. https://doi.org/10.20944/preprints202402.0930.v1
Karaduman, T.; Soylukan, C. Bioactive Compounds from Campsis radicans L.: Antioxidant and Antiproliferative Effects on Colon Cancer Cells. Preprints2024, 2024020930. https://doi.org/10.20944/preprints202402.0930.v1
APA Style
Karaduman, T., & Soylukan, C. (2024). Bioactive Compounds from Campsis radicans L.: Antioxidant and Antiproliferative Effects on Colon Cancer Cells. Preprints. https://doi.org/10.20944/preprints202402.0930.v1
Chicago/Turabian Style
Karaduman, T. and Caner Soylukan. 2024 "Bioactive Compounds from Campsis radicans L.: Antioxidant and Antiproliferative Effects on Colon Cancer Cells" Preprints. https://doi.org/10.20944/preprints202402.0930.v1
Abstract
This study focuses on the potential medicinal properties of Campsis radicans L. extracts and its aim is to investigate the antioxidant and antiproliferative effects of these extracts on the human colon adenocarcinoma cell line (HT-29). Cytotoxic activity in the HT-29 cell line was evaluated, and the 3-dimensional structures of phenolic compounds were obtained using the GC-MS method. Docking simulations were performed with AutoDock Vina, targeting proteins that are crucial in the apoptotic pathway (Bax, Bcl-2, and Caspase-3). Drug similarities and ADMET estimates of the phenolic compounds were obtained from databases. The highest antioxidant activity of Campsis radicans L. plant was observed in ethanol and water extracts, with notable inhibition capacities. While the total phenolic content was highest in ethanol, water, and ether extracts respectively the total flavonoid content was highest in ethanol, then ether, and water extracts. Certain elements were undetectable in mineral determination, and others exceeded the toxic doses for plants. The water extract exhibited dose-dependent cytotoxic activity in the HT-29 cell line, with an IC50 value of 4.47 mg/mL. Molecular docking results revealed the binding of phenolic molecules to the active sites of apoptotic pathway proteins. The findings suggest that Campsis radicans L. plant possesses cytotoxic properties, significant phenolic and flavonoid content, and a high antioxidant capacity. These properties make it a potential candidate for treatment use, emphasizing its potential therapeutic value in the HT-29 cell line. Further research and exploration on the identified phenolic compounds could contribute to the development of novel therapeutic agents.
Keywords
MTT; HT-29; Antioxidant; Antiproliferative; Total phenolic content; Total flavonoid content
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
