Lenart-Boroń, A.; Stankiewicz, K.; Czernecka, N.; Ratajewicz, A.; Bulanda, K.; Heliasz, M.; Sosińska, D.; Dworak, K.; Ciesielska, D.; Siemińska, I.; Tischner, M. Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection. Int. J. Mol. Sci.2024, 25, 3121.
Lenart-Boroń, A.; Stankiewicz, K.; Czernecka, N.; Ratajewicz, A.; Bulanda, K.; Heliasz, M.; Sosińska, D.; Dworak, K.; Ciesielska, D.; Siemińska, I.; Tischner, M. Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection. Int. J. Mol. Sci. 2024, 25, 3121.
Lenart-Boroń, A.; Stankiewicz, K.; Czernecka, N.; Ratajewicz, A.; Bulanda, K.; Heliasz, M.; Sosińska, D.; Dworak, K.; Ciesielska, D.; Siemińska, I.; Tischner, M. Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection. Int. J. Mol. Sci.2024, 25, 3121.
Lenart-Boroń, A.; Stankiewicz, K.; Czernecka, N.; Ratajewicz, A.; Bulanda, K.; Heliasz, M.; Sosińska, D.; Dworak, K.; Ciesielska, D.; Siemińska, I.; Tischner, M. Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection. Int. J. Mol. Sci. 2024, 25, 3121.
Abstract
Skin wounds and their infections by antibiotic resistant bacteria (ARB)are very common in small animals, posing the risk of acquiring ARB by pet owners or antibiotic resistance gene (ARG) transfer to the owners’ microbiota. The aim of this study was to identify the most common pathogens infecting wounds of companion animals, assess their antibiotic resistance and determine the ARGs using culture-based, molecular and proteomic methods. A total of 136 bacterial strains were isolated from wound swabs. Their species was identified using chromogenic media, followed by MALDI-TOF spectrometry. Disk diffusion was used to test the antibiotic resistance and twelve ARGs were detected using PCRs. The dominant species included Staphylococcus pseudintermedius (9.56%), E. coli and E. faecalis (both n=11, 8.09%). Enterobacterales were mostly resistant to amoxicillin/clavulanic acid (68.3% strains), all Pseudomonas were resistant to ceftazidime, piperacillin/tazobactam, imipenem and tylosin, Acinetobacter was mostly resistant to tylosin (55.5%), all Enterococcus were resistant to imipenem, 39.2% of Staphylococci were resistant to clindamycin. Among ARGs, strA (streptomycin resistance), sul3 (sulfonamide resistance) and blaTEM, an ESBL determinant, were the most frequent. The risk of ARB and ARD transfer between animals and humans causes the need to search for new antimicrobial therapies in the future veterinary medicine.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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