Article
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Preserved in Portico This version is not peer-reviewed
Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells
Version 1
: Received: 14 December 2023 / Approved: 14 December 2023 / Online: 15 December 2023 (09:22:50 CET)
A peer-reviewed article of this Preprint also exists.
Pei, Y.A.; Patel, J.; Pei, M. Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells. Appl. Sci. 2024, 14, 1932. Pei, Y.A.; Patel, J.; Pei, M. Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells. Appl. Sci. 2024, 14, 1932.
Abstract
Adult mesenchymal stem cells (MSCs) are a promising cell source for tissue regeneration. However, ex vivo expansion results in cell senescence; cells lose their proliferation and differentiation capacity. Fetal MSCs can be an alternative due to their robust proliferation and differentiation capacities as well as immune privilege properties. Given the rejuvenation effect of decellularized extracellular matrix (dECM) on adult MSCs, it remains unknown whether dECM influences the regenerative potential of fetal stem cells. In this study, passage five fetal nucleus pulposus cells (fNPCs) and fetal synovium-derived stem cells (fSDSCs) were expanded on dECMs deposited by fNPCs (NECM) and fSDSCs (SECM) for one passage with expansion on tissue culture plastic (Plastic) as a control. We found that dECM expanded fNPCs and fSDSCs exhibited both similarities and differences in expression of stemness genes and surface markers. Expanded fNPCs yielded more differentiated pellets after chondrogenic induction but no adipogenic differentiation following adipogenic induction in both Plastic and dECM groups than the corresponding fSDSC group. Despite a significant increase in fNPCs, dECM expanded fSDSCs exhibited no increase in chondrogenic potential; however, compared to the Plastic group, dECM expanded fSDSCs exhibited a small increase in osteogenic potential and a great increase in adipogenic potential. These results suggest that fNPCs are more sensitive to NECM rejuvenation for cartilage tissue engineering and regeneration; in contrast, dECMs exhibited limited effects on fSDSC rejuvenation in chondrogenic capacity except for enhanced adipogenic capacity following expansion on SECM.
Keywords
fetal stem cells; decellularized extracellular matrix; differentiation; fetal nucleus pulposus cell; fetal synovium-derived stem cell
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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