Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Autophagy-Related Musa acuminata Protein MaATG8F Interacts with MaATG4B, Regulating Banana Disease Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

Huoqing Huang
ORCID logo ,
Yuzhen Tian
,
Yile Huo
,
Yushan Liu
,
Wenlong Yang
,
Yuqing Li
,
Mengxia Zhuo
,
Dandan Xiang
ORCID logo ,
Chunyu Li
,
Ganjun Yi
* ,
Siwen Liu
* ORCID logo
Version 1 : Received: 5 December 2023 / Approved: 6 December 2023 / Online: 6 December 2023 (03:17:36 CET)

A peer-reviewed article of this Preprint also exists.

Huang, H.; Tian, Y.; Huo, Y.; Liu, Y.; Yang, W.; Li, Y.; Zhuo, M.; Xiang, D.; Li, C.; Yi, G.; Liu, S. The Autophagy-Related Musa acuminata Protein MaATG8F Interacts with MaATG4B, Regulating Banana Disease Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4. J. Fungi 2024, 10, 91. Huang, H.; Tian, Y.; Huo, Y.; Liu, Y.; Yang, W.; Li, Y.; Zhuo, M.; Xiang, D.; Li, C.; Yi, G.; Liu, S. The Autophagy-Related Musa acuminata Protein MaATG8F Interacts with MaATG4B, Regulating Banana Disease Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4. J. Fungi 2024, 10, 91.

Abstract

Banana is one of the most important fruits in the world due to its status as a major food source for more than 400 million people. Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) causes substantial losses of banana crops every year, and molecular host resistance mechanisms are currently unknown. We here performed a genome-wide analysis of the autophagy related protein 8 (ATG8) family in a wild banana species. The banana genome was found to contain 10 MaATG8 genes. Four MaATG8s formed a gene cluster in the distal part of chromosome 4. Phylogenetic analysis of ATG8 families in banana, Arabidopsis thaliana, Citrus, rice, and ginger revealed five major phylogenetic clades shared by all of these plant species, demonstrating evolutionary conservation of the MaATG8 families. The transcriptomic analysis of plants infected with Foc TR4 showed that almost all of the MaATG8 genes were more highly induced in resistant cultivars than in susceptible cultivars. Finally, MaATG8F was found to interact with MaATG4B in vitro (with yeast two-hybrid assays), and MaATG8F and MaATG4B all positively regulated banana resistance to Foc TR4. Our study provides novel insights into the structure, distribution, evolution, and expression of the MaATG8 family in bananas. Furthermore, the discovery of interactions between MaATG8F and MaATG4 could facilitate future researches of disease resistance genes for genetic improvement of bananas.

Keywords

ATG8; ATG4; autophagy; Fusarium oxysporum f. sp. cubense tropical race 4; plant disease resistance

Subject

Biology and Life Sciences, Horticulture

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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