Dembélé, P.; Cissoko, M.; Diarra, A.Z.; Doumbia, L.; Koné, A.; Magassa, M.H.; Mehadji, M.; Thera, M.A.; Ranque, S. Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis and Mapping of Different Plasmodium Species in Mali. Int. J. Environ. Res. Public Health2024, 21, 228.
Dembélé, P.; Cissoko, M.; Diarra, A.Z.; Doumbia, L.; Koné, A.; Magassa, M.H.; Mehadji, M.; Thera, M.A.; Ranque, S. Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis and Mapping of Different Plasmodium Species in Mali. Int. J. Environ. Res. Public Health 2024, 21, 228.
Dembélé, P.; Cissoko, M.; Diarra, A.Z.; Doumbia, L.; Koné, A.; Magassa, M.H.; Mehadji, M.; Thera, M.A.; Ranque, S. Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis and Mapping of Different Plasmodium Species in Mali. Int. J. Environ. Res. Public Health2024, 21, 228.
Dembélé, P.; Cissoko, M.; Diarra, A.Z.; Doumbia, L.; Koné, A.; Magassa, M.H.; Mehadji, M.; Thera, M.A.; Ranque, S. Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis and Mapping of Different Plasmodium Species in Mali. Int. J. Environ. Res. Public Health 2024, 21, 228.
Abstract
Background: First-line biological diagnosis of malaria in Mali is based on the use of rapid diagnostic tests (RDT), which detect in the blood the Histidin Rich Protein 2 antigen, specific to Plasmodium falciparum. Our study, based on a real-time polymerase chain reaction (qPCR) gold-standard diagnosis, aims to evaluate the performance of RDTs used in Mali and to describe the distribution of Plasmodium species in each administrative regions of Mali; Methods: We collected RDT cassettes in 47 sites of the nine regions of Mali. We randomly selected 150 malaria-negative and up to 30 malaria-positive RDTs from each regions. DNA was extracted from the RDTs’ nitrocellulose strip and then assayed with a pan-Plasmodium qPCR. Positive samples were then analyzed with qPCRs specific for P. falciparum, P. malariae, P. vivax, and P. ovale; Results: Of the 1,389 tested by qPCR RDTs, 258 (18.6%) were positive for Plasmodium spp. P.falciparum was the most predominant (97.3%). However, the prevalence of P.vivax reach 21.1% in the Ménaka region in the north; Conclusion: Overall, RDT diagnostic indices are adequate for the biological diagnosis of malaria in Mali. Our finding support the adjustment of RDTs to the local epidemiology.
Keywords
Diagnostic; Paludisme; TDR; Sensibilité; Spécificité; RAP; Mali
Subject
Public Health and Healthcare, Public Health and Health Services
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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