Version 1
: Received: 25 September 2023 / Approved: 26 September 2023 / Online: 26 September 2023 (11:59:48 CEST)
How to cite:
Colín-Val, Z.; Vega-Miranda, A.; Peralta-Arrieta, I.; Mendoza-Milla, C.; López-Marure, R. Dehydroepiandrosterone Suppresses Proliferation and Migration while Promoting Autophagy in Lung Cancer Cells. Preprints2023, 2023091776. https://doi.org/10.20944/preprints202309.1776.v1
Colín-Val, Z.; Vega-Miranda, A.; Peralta-Arrieta, I.; Mendoza-Milla, C.; López-Marure, R. Dehydroepiandrosterone Suppresses Proliferation and Migration while Promoting Autophagy in Lung Cancer Cells. Preprints 2023, 2023091776. https://doi.org/10.20944/preprints202309.1776.v1
Colín-Val, Z.; Vega-Miranda, A.; Peralta-Arrieta, I.; Mendoza-Milla, C.; López-Marure, R. Dehydroepiandrosterone Suppresses Proliferation and Migration while Promoting Autophagy in Lung Cancer Cells. Preprints2023, 2023091776. https://doi.org/10.20944/preprints202309.1776.v1
APA Style
Colín-Val, Z., Vega-Miranda, A., Peralta-Arrieta, I., Mendoza-Milla, C., & López-Marure, R. (2023). Dehydroepiandrosterone Suppresses Proliferation and Migration while Promoting Autophagy in Lung Cancer Cells. Preprints. https://doi.org/10.20944/preprints202309.1776.v1
Chicago/Turabian Style
Colín-Val, Z., Criselda Mendoza-Milla and Rebeca López-Marure. 2023 "Dehydroepiandrosterone Suppresses Proliferation and Migration while Promoting Autophagy in Lung Cancer Cells" Preprints. https://doi.org/10.20944/preprints202309.1776.v1
Abstract
Lung cancer is a prevalent and aggressive neoplasm worldwide, contributing to significant mortality rates. Dehydroepiandrosterone (DHEA) constitutes the bulk of the steroid hormone in human plasma, has a robust antiproliferative effect, and induces cell death in various tumor cells. However, its role in lung cancer cells remains unexplored. This study aimed to investigate the influence of DHEA on the proliferation, viability, autophagy, and migration of several lung cancer cell lines, including A549, HCC827, and NCI-H2347. Cell proliferation was assessed through crystal violet staining; cell number and viability were evaluated using trypan blue staining; viability was confirmed by MTT reduction, a method that is also an indicator of metabolic activity; migration was assessed via a wound healing assay. Autophagy was evaluated using a specific kit, while cell death was determined by annexin-V-FITC/propidium iodide staining and caspase-3/7 activity assay. The results indicate that DHEA significantly reduced proliferation, cell number, metabolic activity, and migration in all examined lung tumor cells. These effects correlate with an increased autophagy induced by DHEA. No signs of apoptosis or necrosis were observed across the range of DHEA concentrations used. Although these findings are preliminary, they suggest that DHEA could hold promise as an alternative treatment option for various subtypes of lung cancer.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
