Version 1
: Received: 1 September 2023 / Approved: 4 September 2023 / Online: 5 September 2023 (09:39:44 CEST)
How to cite:
Ivkin, A.A.; Grigoriev, E.; Morgun, A.V.; Balakhnin, D.G. The Effects of Transfusion on a Neurovascular Unit in an Experiment Using Its Cellular Model. Preprints2023, 2023090295. https://doi.org/10.20944/preprints202309.0295.v1
Ivkin, A.A.; Grigoriev, E.; Morgun, A.V.; Balakhnin, D.G. The Effects of Transfusion on a Neurovascular Unit in an Experiment Using Its Cellular Model. Preprints 2023, 2023090295. https://doi.org/10.20944/preprints202309.0295.v1
Ivkin, A.A.; Grigoriev, E.; Morgun, A.V.; Balakhnin, D.G. The Effects of Transfusion on a Neurovascular Unit in an Experiment Using Its Cellular Model. Preprints2023, 2023090295. https://doi.org/10.20944/preprints202309.0295.v1
APA Style
Ivkin, A.A., Grigoriev, E., Morgun, A.V., & Balakhnin, D.G. (2023). The Effects of Transfusion on a Neurovascular Unit in an Experiment Using Its Cellular Model. Preprints. https://doi.org/10.20944/preprints202309.0295.v1
Chicago/Turabian Style
Ivkin, A.A., Andrey V. Morgun and Dmitry G. Balakhnin. 2023 "The Effects of Transfusion on a Neurovascular Unit in an Experiment Using Its Cellular Model" Preprints. https://doi.org/10.20944/preprints202309.0295.v1
Abstract
Purpose of the study: to reveal how the transfusion and the refraining from it affects the neuro-vascular unit in the congenital heart defects correction with cardiopulmonary bypass. Materials and methods: in vitro research, we formed a model of a neurovascular unit, which contains neu-rons, astrocytes and endothelial cells. This cellular model was cultivated in hypoxia conditions with the oxygen saturation levels of 1, 2, 3, 4%. In addition, this unit was cultivated with the pa-tient's serum adding in two different groups - with minimal and maximal levels of system in-flammatory response that we estimated by IL-6 level. Results: We found that during incubation in hypoxic conditions, the level of transendothelial resistance changed in groups with oxygen levels of 1% and 2% only after 4 hours. When we cultivated neurovascular unit cells with patients' sera, we found a minimal level of transendothelial resistance at 4 hours that increased after 24 hours. Permeability analysis showed that its level significantly decreased in the 1% and 2% oxygen group at 60- and 90-minutes relative to the control group. Conclusion: the system inflammatory response has more expressed but less long effect on the neurovascular unit model than hypoxia.
Keywords
children; systemic inflammatory response; cardiac surgery; neurovascular unit; critical care medicine
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
