Version 1
: Received: 3 August 2023 / Approved: 4 August 2023 / Online: 4 August 2023 (11:43:13 CEST)
How to cite:
Khan, S.; Akhtar, N.; Rha, E.S.; Rehman, S.U.; Jamil, M. Bacillus Subtilus Synthesized Iron Oxide Nanoparticles Induced Protein and Gene’s Expression Profile of Rice (Oryza sativa L.) Plant. Preprints2023, 2023080402. https://doi.org/10.20944/preprints202308.0402.v1
Khan, S.; Akhtar, N.; Rha, E.S.; Rehman, S.U.; Jamil, M. Bacillus Subtilus Synthesized Iron Oxide Nanoparticles Induced Protein and Gene’s Expression Profile of Rice (Oryza sativa L.) Plant. Preprints 2023, 2023080402. https://doi.org/10.20944/preprints202308.0402.v1
Khan, S.; Akhtar, N.; Rha, E.S.; Rehman, S.U.; Jamil, M. Bacillus Subtilus Synthesized Iron Oxide Nanoparticles Induced Protein and Gene’s Expression Profile of Rice (Oryza sativa L.) Plant. Preprints2023, 2023080402. https://doi.org/10.20944/preprints202308.0402.v1
APA Style
Khan, S., Akhtar, N., Rha, E.S., Rehman, S.U., & Jamil, M. (2023). Bacillus Subtilus Synthesized Iron Oxide Nanoparticles Induced Protein and Gene’s Expression Profile of Rice (Oryza sativa L.) Plant. Preprints. https://doi.org/10.20944/preprints202308.0402.v1
Chicago/Turabian Style
Khan, S., Shafiq Ur Rehman and Muhammad Jamil. 2023 "Bacillus Subtilus Synthesized Iron Oxide Nanoparticles Induced Protein and Gene’s Expression Profile of Rice (Oryza sativa L.) Plant" Preprints. https://doi.org/10.20944/preprints202308.0402.v1
Abstract
Arsenic (As) is the most poisonous metalloid with negative effects on the plant and ecosystem. In the current study, observed that iron oxide nanoparticles (NPs) affected the molecular features of rice under arsenic (As) stress. The result showed that 1-D electrophoresis (SDS-PAGE) demonstrated variable expression of protein profiles. The polypeptide pattern was altered in the SDS-PAGE under arsenic stress. The findings also showed that low molecular weight polypeptides (MTs) expression was maximum under arsenic stress, while iron oxide NPs decreased the expression of these polypeptides and reduced the arsenic stress. RT-PCR was used to analyse the expression of the MYB transcriptional factor, WRKY, and OsGF14 genes in the rice plant. The results showed that these genes were up-regulated in response to arsenic stress, iron oxide NPs reduced arsenic stress, and Myeloblastosis (MYB), WRKY, and OsGF14 genes had very low expression levels. Our work showed that iron oxide nanoparticles (NPs) not only reduced the effects of arsenic stress but also decreased the expression of stress-related genes and metallothioneins (MTs).
Keywords
arsenic; iron oxide NPs; transcriptional factor; gene; SDS-PAGE
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
